Li JiaPing, Habibovic Pamela, Yuan Huipin, van den Doel Mirella, Wilson Clayton E, de Wijn Joost R, van Blitterswijk Clemens A, de Groot Klass
Institute for Biomedical Technology, University of Twente, The Netherlands.
Biomaterials. 2007 Oct;28(29):4209-18. doi: 10.1016/j.biomaterials.2007.05.042. Epub 2007 Jul 5.
In this study, porous 3D fiber deposition titanium (3DFT) and 3DFT combined with porous biphasic calcium phosphate ceramic (3DFT+BCP) implants, both bare and 1 week cultured with autologous bone marrow stromal cells (BMSCs), were implanted intramuscularly and orthotopically in 10 goats. To assess the dynamics of bone formation over time, fluorochrome markers were administered at 3, 6 and 9 weeks and the animals were sacrificed at 12 weeks after implantation. New bone in the implants was investigated by histology and histomorphometry of non-decalcified sections. Intramuscularly, no bone formation was found in any of the 3DFT implants, while a very limited amount of bone was observed in 2 BMSC 3DFT implants. 3DFT+BCP and BMSC 3DFT+BCP implants showed ectopic bone formation, in 8 and 10 animals, respectively. The amount of formed bone was significantly higher in BMSC 3DFT+BCP as compared to 3DFT+BCP implants. Implantation on transverse processes resulted in significantly more bone formation in composite structure as compared to titanium alloy alone, both with and without cells. Unlike intramuscularly, the presence of BMSC did not have a significant effect on the amount of new bone either in metallic or in composite structure. Although the 3DFT is inferior to BCP for bone growth, the reinforcement of the brittle BCP with a 3DFT cage did not negatively influence osteogenesis, osteoinduction and osteoconduction as previously shown for the BCP alone. The positive effect of BMSCs was observed ectopically, while it was not significant orthotopically.
在本研究中，将多孔三维纤维沉积钛（3DFT）以及与多孔双相磷酸钙陶瓷联合的3DFT（3DFT+BCP）植入物（均为裸植入物以及用自体骨髓基质细胞（BMSC）培养1周后的植入物）肌肉内和原位植入10只山羊体内。为了评估随时间推移骨形成的动态变化，在植入后3周、6周和9周给予荧光染料标记物，并在植入后12周处死动物。通过对未脱钙切片进行组织学和组织形态计量学研究植入物中的新骨。在肌肉内，任何3DFT植入物中均未发现骨形成，而在2个BMSC 3DFT植入物中观察到极少量的骨。3DFT+BCP和BMSC 3DFT+BCP植入物分别在8只和10只动物中显示出异位骨形成。与3DFT+BCP植入物相比，BMSC 3DFT+BCP中形成的骨量显著更高。与单独的钛合金相比，无论有无细胞，横突植入复合结构时均导致显著更多的骨形成。与肌肉内植入不同，BMSC的存在对金属或复合结构中的新骨量均无显著影响。尽管3DFT在促进骨生长方面不如BCP，但用3DFT笼加强脆性BCP并未如先前单独BCP那样对成骨、骨诱导和骨传导产生负面影响。BMSC的积极作用在异位时观察到，而在原位时不显著。
