Fellah Borhane H, Gauthier Olivier, Weiss Pierre, Chappard Daniel, Layrolle Pierre
INSERM U791, Laboratory for Osteoarticular and Dental Tissue Engineering, Faculty of Dental Surgery, 1 Place Alexis Ricordeau, Nantes, France.
Biomaterials. 2008 Mar;29(9):1177-88. doi: 10.1016/j.biomaterials.2007.11.034.
The aim of this work was to compare the osteogenicity of calcium phosphate ceramic granules with autologous bone graft in ectopic and orthotopic sites. Biphasic calcium phosphate (BCP) granules composed of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) in a 60/40 ratio were sintered at 1050, 1125 and 1200 degrees C, producing different microporosities. Either BCP ceramic granules or autologous bone chips (n=7) were implanted into paraspinal muscles. Osteoinduction was not observed in either the BCP implants or autologous bone chips after 6 or 12 weeks in the ectopic sites. Hollow and bored polytetrafluoroethylene (PTFE) cylinders were filled with autologous bone, BCP granules or left empty, then implanted into critical-sized defects in femoral epiphyses. The PTFE cylinders left empty contained marrow and blood vessels but not mineralized bone, indicating that this model prevented bone ingrowth (0.56+/-0.43% at 12 weeks). Bone formation was observed in contact with the BCP1050 and BCP1125 granules in the femoral sites after 6 weeks. The amount of bone after 12 weeks was 5.6+/-7.3 and 9.6+/-6.6% for BCP1050 and BCP1125, respectively. Very little bone formation was observed with the BCP1200 implants (1.5+/-1.3% at 12 weeks). In both the ectopic and orthotopic sites, autologous bone chips were drastically resorbed (from 19.4+/-3.7% initially to 1.7+/-1.2% at 12 weeks). This study shows that synthetic bone substitutes may have superior stability and osteogenic properties than autologous bone grafts in critical-sized bone defects.
本研究的目的是比较磷酸钙陶瓷颗粒与自体骨移植在异位和原位部位的成骨能力。由羟基磷灰石（HA）和β-磷酸三钙（β-TCP）按60/40比例组成的双相磷酸钙（BCP）颗粒在1050、1125和1200℃下烧结，产生不同的微孔率。将BCP陶瓷颗粒或自体骨碎片（n = 7）植入椎旁肌。在异位部位植入BCP或自体骨碎片6周或12周后，均未观察到骨诱导现象。将中空且有孔的聚四氟乙烯（PTFE）圆柱体填充自体骨、BCP颗粒或保持空的状态，然后植入股骨骨骺的临界尺寸缺损处。空的PTFE圆柱体含有骨髓和血管，但没有矿化骨，表明该模型可防止骨长入（12周时为0.56±0.43%）。6周后在股骨部位观察到与BCP1050和BCP1125颗粒接触处有骨形成。12周时，BCP1050和BCP1125的骨量分别为5.6±7.3%和9.6±6.6%。BCP1200植入物观察到的骨形成很少（12周时为1.5±1.3%）。在异位和原位部位，自体骨碎片均被大量吸收（从最初的19.4±3.7%降至12周时的1.7±1.2%）。本研究表明，在临界尺寸骨缺损中，合成骨替代物可能比自体骨移植具有更好的稳定性和成骨特性。
