Yamada Paulette M, Amorim Fabiano T, Moseley Pope, Robergs Robert, Schneider Suzanne M
Department of Physical Performance and Development, University of New Mexico, Albuquerque, New Mexico, USA.
J Appl Physiol (1985). 2007 Oct;103(4):1196-204. doi: 10.1152/japplphysiol.00242.2007. Epub 2007 Jul 5.
Heat acclimation (HA) results in whole body adaptations that increase heat tolerance, and in addition, HA may also result in protective cellular adaptations. We hypothesized that, after HA, basal intracellular heat shock protein (HSP) 72 and extracellular IL-10 levels would increase, while extracellular HSP72 levels decrease. Ten male and two female subjects completed a 10-day exercise/HA protocol (100-min exercise bout at 56% of maximum O(2) uptake in a 42.5 degrees C DB, 27.9% RH environment); subjects exhibited classic adaptations that accompany HA. Peripheral blood mononuclear cells (PBMCs) were isolated before and after each acclimation session on days 1, 6, and 10; plasma and serum were collected before and after exercise on the 1st and 10th day of HA. SDS-PAGE was used to determine PBMC HSP72 levels during HA, and ELISA was used to measure plasma IL-10 and serum HSP72 concentrations. The increase in PBMC HSP72 from pre- to postexercise on the 1st day of HA was not significant (mean +/- SD, 1.0 +/- 0 vs. 1.6 +/- 0.6 density units). Preexercise HSP72 levels on day 1 were significantly lower compared with the pre- and postexercise samples on days 6 and 10 (mean +/- SD, day 6: 2.1 +/- 1.0 and 2.2 +/- 1.0, day 10: 2.0 +/- 1.3 and 2.2 +/- 1.0 density units, respectively, P < 0.05). There were no differences in plasma IL-10 and serum HSP72 postexercise or after 10 days of HA. The sustained elevation of HSP72 from days 6 to 10 may be evidence of a cellular adaptation to HA that contributes to improved heat tolerance and reduced heat illness risk.
热适应（HA）会导致全身适应性变化，从而提高耐热性，此外，热适应还可能导致细胞产生保护性适应。我们推测，热适应后，基础细胞内热休克蛋白（HSP）72和细胞外白细胞介素-10水平会升高，而细胞外HSP72水平会降低。10名男性和2名女性受试者完成了一项为期10天的运动/热适应方案（在42.5摄氏度干球温度、27.9%相对湿度环境中，以最大摄氧量的56%进行100分钟运动 bout）；受试者表现出了热适应伴随的典型适应性变化。在热适应第1天、第6天和第10天的每次适应训练前后，分离外周血单个核细胞（PBMC）；在热适应第1天和第10天运动前后采集血浆和血清。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定热适应期间PBMC的HSP72水平，采用酶联免疫吸附测定（ELISA）测量血浆白细胞介素-10和血清HSP72浓度。热适应第1天运动前至运动后PBMC中HSP72的增加不显著（平均值±标准差，1.0±0对1.6±0.6密度单位）。第1天运动前HSP72水平显著低于第6天和第10天运动前和运动后的样本（平均值±标准差，第6天：2.1±1.0和2.2±1.0，第10天：2.0±1.3和2.2±1.0密度单位，P<0.05）。运动后或热适应10天后，血浆白细胞介素-10和血清HSP72没有差异。从第6天到第10天HSP72的持续升高可能是细胞对热适应的一种适应证据，有助于提高耐热性并降低中暑风险。
