Fehrenbach E, Niess A M, Veith R, Dickhuth H H, Northoff H
Department of Transfusion Medicine, Department of Sports Medicine, University of Tuebingen, Tuebingen, Germany.
J Leukoc Biol. 2001 May;69(5):747-54.
Overexpression of the heat shock protein HSP72 provides thermotolerance. We asked if two consecutive endurance runs 1 week apart (CR1, CR2) and additional environmental heat stress affect HSP72-expression in leukocytes of nonheat-acclimated endurance athletes. Twelve subjects were allocated randomly into two groups. Group HH completed both runs at 28 degrees C ambient temperature, and group NH performed CR1 at 18 degrees C and CR2 at 28 degrees C. HSP72-expression was determined by flow cytometry and RT-PCR before and 0, 24, and 48 h after exercise. Additionally, post-exercise cells were exposed to in vitro heat shock (HS; 2 h, 42 degrees C). The prolonged, high HSP72 protein level after CR1 in HH compared with NH may reflect thermotolerance induced by endurance exercise at high ambient temperature. Adaptation of cardiocirculatory/thermoregulatory capacity after CR2 in HH went along with a more rapid down-regulation of HSP72 compared with CR1. HSP72 mRNA demonstrated temperature-related changes after exercise. The reduced HS response in vitro after CR2 may represent exercise-related adaptation mechanisms. HSP72 concentrations in leukocytes may indicate previous exercise- and temperature-related stress conditions and adaptation in immunocompetent cells.
热休克蛋白HSP72的过表达可提供耐热性。我们研究了间隔1周进行的两次连续耐力跑（CR1、CR2）以及额外的环境热应激是否会影响未进行热适应的耐力运动员白细胞中HSP72的表达。12名受试者被随机分为两组。HH组在28摄氏度的环境温度下完成两次跑步，NH组在18摄氏度下进行CR1，在28摄氏度下进行CR2。在运动前以及运动后0、24和48小时，通过流式细胞术和逆转录聚合酶链反应测定HSP72的表达。此外，运动后的细胞接受体外热休克（HS；2小时，42摄氏度）。与NH组相比，HH组在CR1后HSP72蛋白水平持续升高，这可能反映了在高环境温度下耐力运动诱导的耐热性。与CR1相比，HH组在CR2后心脏循环/体温调节能力的适应伴随着HSP72更快的下调。运动后HSP72 mRNA表现出与温度相关的变化。CR2后体外HS反应的降低可能代表了与运动相关的适应机制。白细胞中HSP72的浓度可能表明先前与运动和温度相关的应激条件以及免疫活性细胞中的适应性。
