[缺氧促进大鼠睾丸生殖细胞凋亡]

[Hypoxia promotes apoptosis of germ cells in rat testes].

作者信息

Liao Wei-gong, Gao Yu-qi, Cai Ming-chun, Wu Yi, Huang Jian, Fan You-ming

机构信息

Department of Pathophysiology and High Altitude Physiology, Third Military Medical University, Chongqing, 400038, China.

出版信息

Zhonghua Nan Ke Xue. 2007 Jun;13(6):487-91.

PMID:17615969

Abstract

OBJECTIVE

To explore the effects of hypobaric hypoxia on the apoptosis of germ cells in male rats.

METHODS

Adult male Wistar rats were randomly divided into four groups: a control group raised at sea level; a 5 d, a 15 d and a 30 d hypoxic group raised in a hypobaric chamber simulating 5000 m altitude for 5 days, 15 days and 30 days respectively. Flow cytometry and TUNEL were used to evaluate the apoptosis of germ cells in the testis. Bax and Bcl-2 in the testis were measured by Western blot.

RESULTS

Seminiferous tubules with apoptotic germ cells were significantly more in the hypoxic groups than in the control (P < 0.01). Most apoptotic germ cells were spermatogonia and spermatocytes. Compared with the control group, apoptotic germ cells detected by PI flow cytometry were significantly increased in the hypoxic 15 d and 30 d groups (P < 0.05); Bax was significantly higher (P < 0.05), and so was the ratio of Bax to Bcl-2 in the hypoxic 30 d group (P < 0.01).

CONCLUSION

Hypoxia promotes apoptosis of testicular germ cells in male rats. Chronic hypoxia increases Bax expression in the rat testis.

摘要

目的

探讨低压低氧对雄性大鼠生殖细胞凋亡的影响。

方法

成年雄性Wistar大鼠随机分为四组：对照组饲养于海平面；5天、15天和30天低压低氧组分别在模拟海拔5000米的低压舱中饲养5天、15天和30天。采用流式细胞术和TUNEL法评估睾丸生殖细胞凋亡情况。通过蛋白质免疫印迹法检测睾丸中Bax和Bcl-2的表达。

结果

低压低氧组出现凋亡生殖细胞的生精小管明显多于对照组（P<0.01）。多数凋亡生殖细胞为精原细胞和精母细胞。与对照组相比，低压低氧15天和30天组经PI流式细胞术检测的凋亡生殖细胞显著增加（P<0.05）；低压低氧30天组Bax显著升高（P<0.05），Bax与Bcl-2的比值也显著升高（P<0.01）。