德国南部伯氏疏螺旋体复合群的流行病学特征及分子特性，特别关注新物种斯氏疏螺旋体（Borrelia spielmanii sp. nov.）

Epidemiological aspects and molecular characterization of Borrelia burgdorferi s.l. from southern Germany with special respect to the new species Borrelia spielmanii sp. nov.

作者信息

Fingerle Volker, Schulte-Spechtel Ulrike C, Ruzic-Sabljic Eva, Leonhard Sarah, Hofmann Heidelore, Weber Klaus, Pfister Kurt, Strle Franc, Wilske Bettina

机构信息

National Reference Center for Borreliae, Max von Pettenkofer-Institut für Medizinische Mikrobiologie und Hygiene der Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, D-80336 Munich, Germany.

出版信息

Int J Med Microbiol. 2008 Apr;298(3-4):279-90. doi: 10.1016/j.ijmm.2007.05.002. Epub 2007 Jul 5.

DOI:10.1016/j.ijmm.2007.05.002

PMID:17616434

Abstract

In 475 Borrelia-infected Ixodes ricinus (2155 ticks investigated) from southern Germany the most common Borrelia burgdorferi sensu lato species was B. garinii (34.3%) followed by B. afzelii (25.1%), B. burgdorferi sensu stricto (22.0%), and B. valaisiana (12.7%). B. spielmanii sp. nov. was detected in 5.9% of the 475 infected ticks. Hints for a focal distribution were found for B. spielmanii sp. nov. and B. garinii OspA type 4. In 242 patient isolates, dominance (66.9%) of B. afzelii for skin could be confirmed, while frequency of B. garinii in cerebrospinal fluid (CSF) isolates (51.1%) was comparable to the frequency in nymphal ticks (51.6%). Four patient isolates from southern Germany and two from Slovenia, all isolated from erythema migrans, could be assigned to B. spielmanii sp. nov. Within this new species high sequence identities were found for rrs, fla, and ospA while rrf-rrl, ospC, and dbpA were less conserved: three new ospC and two new dbpA sequence types were found. This genetic heterogeneity reveals that B. spielmanii sp. nov. did not evolve just recently.

摘要

在德国南部采集的475只感染伯氏疏螺旋体的蓖麻硬蜱（共检测2155只蜱）中，最常见的狭义伯氏疏螺旋体物种是伽氏疏螺旋体（34.3%），其次是阿氏疏螺旋体（25.1%）、狭义伯氏疏螺旋体（22.0%）和瓦莱疏螺旋体（12.7%）。在这475只感染蜱中，5.9%检测到施氏疏螺旋体新种。发现施氏疏螺旋体新种和伽氏疏螺旋体OspA 4型有局灶性分布迹象。在242例患者分离株中，可确认阿氏疏螺旋体在皮肤感染中占主导地位（66.9%），而伽氏疏螺旋体在脑脊液分离株中的频率（51.1%）与若蜱中的频率（51.6%）相当。来自德国南部的4例患者分离株和来自斯洛文尼亚的2例患者分离株，均从游走性红斑中分离得到，可归为施氏疏螺旋体新种。在这个新物种中，rrs、fla和ospA具有较高的序列同一性，而rrf - rrl、ospC和dbpA的保守性较低：发现了3种新的ospC序列类型和2种新的dbpA序列类型。这种遗传异质性表明施氏疏螺旋体新种并非近期才进化形成。