使用一种产生耐热性多磷酸激酶的大肠杆菌重组体作为ATP再生剂来生产1,6-二磷酸果糖。
Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.
作者信息
Iwamoto Seishi, Motomura Kei, Shinoda Yasuharu, Urata Masaaki, Kato Junichi, Takiguchi Noboru, Ohtake Hisao, Hirota Ryuichi, Kuroda Akio
机构信息
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
出版信息
Appl Environ Microbiol. 2007 Sep;73(17):5676-8. doi: 10.1128/AEM.00278-07. Epub 2007 Jul 6.
Abstract
Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.
摘要
经热处理的大肠杆菌产生嗜热栖热菌多聚磷酸激酶，其利用外源多聚磷酸再生ATP。这种重组体可作为一个平台，与耐热磷酸化酶或需能酶结合用于生产有价值的化合物。在这项工作中，我们展示了由果糖和多聚磷酸生产1,6-二磷酸果糖。
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