Ball H J, Finlay D, Mackie D P, Greer D, Pollock D, McNair J
Veterinary Research Laboratories, Stormont, Belfast, United Kingdom.
J Clin Microbiol. 1991 Aug;29(8):1625-8. doi: 10.1128/jcm.29.8.1625-1628.1991.
A monoclonal antibody to a 23.5-kDa bovine inflammatory antigen present in high levels in mastitic milk has been used in an antigen-capture enzyme-linked immunosorbent assay (ELISA) to screen milk samples from herds of cattle for subclinical mastitis. The results from 20 herds with a total of 2,612 quarter samples are presented. Good correlation was observed between the ELISA level and the milk cell count (MCC). The results demonstrated an average of 5% false negatives (1.8% associated with isolates of Staphylococcus aureus and/or Streptococcus spp.) and 7.7% false positives for each herd in relation to mastitic (greater than 400,000 cells per ml) or nonmastitic (less than 400,000 cells per ml) MCCs.
一种针对存在于患乳房炎奶牛乳汁中、含量很高的23.5 kDa牛炎症抗原的单克隆抗体，已被用于抗原捕获酶联免疫吸附测定（ELISA），以筛查牛群的乳汁样本是否患有亚临床乳房炎。本文给出了来自20个牛群、共2612份乳房样本的检测结果。ELISA水平与乳汁细胞计数（MCC）之间观察到良好的相关性。结果表明，相对于患乳房炎（每毫升大于400,000个细胞）或未患乳房炎（每毫升小于400,000个细胞）的MCC，每个牛群平均有5%的假阴性（1.8%与金黄色葡萄球菌和/或链球菌属的分离株有关）和7.7%的假阳性。
