Rios Domingos L S, D'Onofrio Lorenza O, Cerqueira Caio C S, Bonfim-Silva Ricardo, Carvalho Heitor G, Santos-Filho Ademar, Galvão-Castro Bernardo
Laboratório de Genética Molecular, Universidade Estadual do Sudoeste da Bahia (UESB), Jequié, BA, Brazil.
Clin Chem Lab Med. 2007;45(7):874-8. doi: 10.1515/CCLM.2007.136.
Paraoxonases (PON) are members of an enzyme family involved in preventing low-density lipoprotein oxidation and therefore protecting against atherosclerotic plaque formation.
We studied the Met55Leu and Gln192Arg PON1 polymorphisms in 712 patients (437 Caucasian- and 275 African-Brazilians) who underwent coronary angiography.
Among Caucasian-Brazilians, the homozygous 55LeuLeu frequency was higher among patients with significant coronary artery disease (CAD, obstructive lesions >/=50%) than among lesion-free controls (51% vs. 30.3%; p=0.022) in females, but not in males. The Gln192Arg PON1 polymorphism was not associated with CAD, although 192GlnGln homozygotes presented lower high-density lipoprotein (HDL)-cholesterol (p=0.035) and higher triglyceride (p=0.012) levels than 192Arg allele carriers among Caucasian-Brazilian males, but not females. No other lipid-genotype association was detected. Multivariate logistic regression corrected for classic CAD risk factors shows that 55LeuLeu PON1 homozygotes were at increased CAD risk (odds ratio OR=2.852; p=0.003) and that this genotype interacted with gender in its association with CAD risk (OR=0.290; p=0.006) among Caucasian-Brazilians.
This report shows that the 55LeuLeu PON1 genotype increases CAD risk among female Caucasian-Brazilians, irrespective of other CAD risk factors. In addition, 192GlnGln PON1 homozygotes show higher triglyceride and lower HDL-cholesterol levels in male Caucasian-Brazilians. No associations were detected among African-Brazilians.
对氧磷酶（PON）是一个酶家族的成员，参与防止低密度脂蛋白氧化，从而预防动脉粥样硬化斑块形成。
我们研究了712例接受冠状动脉造影的患者（437名白种巴西人和275名非洲裔巴西人）的Met55Leu和Gln192Arg PON1基因多态性。
在白种巴西女性中，患有严重冠状动脉疾病（CAD，阻塞性病变≥50%）的患者中纯合子55LeuLeu频率高于无病变对照组（51%对30.3%；p=0.022），而男性中则不然。尽管在白种巴西男性而非女性中，192GlnGln纯合子的高密度脂蛋白（HDL）胆固醇水平较低（p=0.035）且甘油三酯水平较高（p=0.012），但Gln192Arg PON1基因多态性与CAD无关。未检测到其他脂质-基因型关联。校正经典CAD危险因素后的多变量逻辑回归显示，在白种巴西人中，55LeuLeu PON1纯合子患CAD的风险增加（优势比OR=2.852；p=0.003），且该基因型在与CAD风险的关联中与性别存在相互作用（OR=0.290；p=0.006）。
本报告表明，55LeuLeu PON1基因型增加了白种巴西女性患CAD的风险，与其他CAD危险因素无关。此外，在白种巴西男性中，192GlnGln PON1纯合子的甘油三酯水平较高，HDL胆固醇水平较低。在非洲裔巴西人中未检测到关联。
