Guan Hao, Zhao Zhijing, He Fei, Zhou Qin, Meng Qingyan, Zhu Xiongxiang, Zheng Zhao, Hu Dahai, Chen Bi
Department of Burn, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, PR China.
Cryobiology. 2007 Aug;55(1):52-9. doi: 10.1016/j.cryobiol.2007.05.001. Epub 2007 Jun 2.
The purpose of present study is to investigate the effects of two different thawing temperatures on normal human fibroblast which dealt with -20 degrees C, hoping to provide a clue for further study in reducing excessive collagen formation after cryotherapy on skin diseases in vitro, as well as in differentiation disorders. In order to elucidate its action mechanism, a programmable freezing device was developed to apply freezing temperatures on cell cultures. The effects of two different thawing temperatures on frozen fibroblast proliferation, viability, collagen synthesis and alpha smooth muscle actin (alpha-SMA) expressing were investigated. We found that compared with 37 degrees C, thawing with 20 degrees C yielded same motility. But there are significant differences in terms of the alpha-SMA expression (P<0.05) of fibroblast and collagen I, III synthesis (P<0.01) between two groups after 72h. The results suggest that comparing with slow thawing; rapid thawing cannot only keep the same cell's damage, but also can modify collagen synthesis and differentiation of fibroblasts. It may be more suitable for the cryosurgical treatment of keloids and benign skin diseases.
本研究的目的是探究两种不同解冻温度对在-20℃处理过的正常人成纤维细胞的影响，希望为进一步研究减少体外冷冻治疗皮肤病后过度胶原蛋白形成以及分化障碍提供线索。为阐明其作用机制，开发了一种可编程冷冻装置以对细胞培养物施加冷冻温度。研究了两种不同解冻温度对冷冻成纤维细胞增殖、活力、胶原蛋白合成和α平滑肌肌动蛋白（α-SMA）表达的影响。我们发现，与37℃解冻相比，20℃解冻产生相同的活力。但72小时后两组之间成纤维细胞的α-SMA表达（P<0.05）以及I、III型胶原蛋白合成（P<0.01）存在显著差异。结果表明，与缓慢解冻相比，快速解冻不仅能保持相同的细胞损伤，还能改变成纤维细胞的胶原蛋白合成和分化。它可能更适合瘢痕疙瘩和良性皮肤病的冷冻手术治疗。
