Hugentobler S A, Morris D G, Sreenan J M, Diskin M G
Animal Production Research Centre, Mellows Campus, Athenry, Co Galway, Ireland.
Theriogenology. 2007 Sep 1;68(4):538-48. doi: 10.1016/j.theriogenology.2007.04.049. Epub 2007 Jul 6.
In the bovine up to 40% of embryos die before implantation but despite the importance of ions in oviduct and uterine fluid formation and in gamete, zygote and early embryo development there is very little published information on the ion concentrations of oviduct or uterine fluid. The free anions chloride, phosphate and sulphate and the free cations sodium, calcium, magnesium and potassium were measured in oviduct fluid on days 0, 2, 4 and 6 and in uterine fluid on days 6, 8 and 14 and in corresponding blood samples. Oviduct and uterine fluids were collected in situ. Sodium was 25-fold higher than potassium and 80-fold higher than the other ions and chloride was 10-fold higher than potassium and 40-fold higher than the other ions in oviduct and uterine fluid. Phosphate, sulphate, magnesium, potassium and calcium were at lower concentrations in all fluids. Oviduct calcium and sodium were higher on day 0 than other days. The most striking uterine differences were the higher potassium and lower chloride, sodium and magnesium on day 14 than other days. There were significant positive associations between oviduct and blood chloride, sulphate, magnesium and calcium while only uterine sulphate was positively related to its blood concentration. There was no relationship between fluid secretion rate and no association between the concentrations of systemic progesterone or oestradiol and any ion in oviduct or uterine fluid. The different concentrations and associations between ions in the oviduct, uterus and blood suggest a differential regulation of ion secretion by the oviduct and uterine epithelia.
在牛中，高达40%的胚胎在着床前死亡。尽管离子在输卵管和子宫液的形成以及配子、合子和早期胚胎发育中起着重要作用，但关于输卵管或子宫液中离子浓度的公开信息却非常少。在第0、2、4和6天测量输卵管液中的游离阴离子氯、磷酸盐和硫酸盐以及游离阳离子钠、钙、镁和钾，在第6、8和14天测量子宫液中的这些离子，并采集相应的血液样本。输卵管液和子宫液在原位采集。在输卵管和子宫液中，钠的含量比钾高25倍，比其他离子高80倍；氯的含量比钾高10倍，比其他离子高40倍。磷酸盐、硫酸盐、镁、钾和钙在所有液体中的浓度较低。输卵管中的钙和钠在第0天比其他日子更高。子宫最显著的差异是第14天的钾含量较高，而氯、钠和镁含量低于其他日子。输卵管液与血液中的氯、硫酸盐、镁和钙之间存在显著的正相关，而只有子宫液中的硫酸盐与其血液浓度呈正相关。液体分泌速率与全身孕酮或雌二醇浓度与输卵管或子宫液中任何离子之间均无关联。输卵管、子宫和血液中离子的不同浓度及相关性表明，输卵管和子宫上皮对离子分泌存在差异调节。
