Grippo A A, Henault M A, Anderson S H, Killian G J
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
J Dairy Sci. 1992 Jan;75(1):58-65. doi: 10.3168/jds.S0022-0302(92)77738-8.
To detect variations in oviduct fluid cation concentrations, Ca++, Mg++, K+, and Na+ were determined for daily samples of blood serum and bovine oviduct fluid collected from indwelling isthmic and ampullary catheters. Isthmic oviduct fluid Ca++ concentration was significantly greater than that in ampullary fluid, particularly around estrus and ovulation. Maximum Ca++ concentrations found in isthmic oviduct fluid at estrus (2.57 +/- .22 mM) and at ovulation (2.50 +/- .29 mM) were similar to those of medium used for in vitro capacitation of bovine sperm. Concentrations of Mg++ in oviduct fluid differed significantly by estrous cycle stage, but not by oviduct region, and were consistently lower than those detected in serum. No relationships were found for K+ or Na+ with respect to region or stage, but K+ was generally higher in oviduct fluid than in serum. The concentration of K+ averaged over stage and region (4.46 +/- .13 mM) and the K+:Na+ ratio (.032 +/- .002) were similar to those reported in bovine in vitro capacitating and fertilizing media. Concentrations of Ca++ and Na+ from peritoneal fluid from nonstaged cows were similar to those of oviduct fluid or serum. The Mg++ concentration was greater, and K+ concentration was less, in peritoneal than in oviduct fluid.
为检测输卵管液中阳离子浓度的变化，对从留置的峡部和壶腹部导管采集的血清和牛输卵管液每日样本测定了Ca++、Mg++、K+和Na+。峡部输卵管液中的Ca++浓度显著高于壶腹部液体，尤其是在发情期和排卵期前后。发情期（2.57±0.22 mM）和排卵期（2.50±0.29 mM）峡部输卵管液中发现的最大Ca++浓度与用于牛精子体外获能的培养基中的浓度相似。输卵管液中Mg++的浓度因发情周期阶段不同而有显著差异，但不因输卵管区域而异，且始终低于血清中检测到的浓度。未发现K+或Na+与区域或阶段有关，但输卵管液中的K+通常高于血清中的K+。跨阶段和区域平均的K+浓度（4.46±0.13 mM）和K+:Na+比值（0.032±0.002）与牛体外获能和受精培养基中报道的相似。未分阶段奶牛的腹膜液中Ca++和Na+的浓度与输卵管液或血清中的相似。腹膜液中的Mg++浓度高于输卵管液，而K+浓度低于输卵管液。
