Starska Katarzyna, Stasikowska Olga, Lukomski Marek, Lewy-Trenda Iwona
Uniwersytet Medyczny w Lodzi, Katedra Otolaryngologii, Klinika Laryngologii Onkologicznej.
Pol Merkur Lekarski. 2007 Sep;23(135):188-91.
Matrix metalloproteases (MMPs) are proteolytic enzymes contribute to tumor expansion by degrading components of the extracellular matrix. MPPs play a key role in tumor invasion and metastases of various cancers and head and neck carcinoma as well. The aim of this study was to investigate MT1-MMP expression in squamous cell carcinoma of the larynx to relate these levels of expression to clinicohistological features of the tumor and lymph nodes e.g. TNM, nodal micrometastases, tumor front grading and 3- and 5-year survival.
The study included 22 patients with laryngeal cancer surgical treated in ENT Department Medical University of Lódź between 1998 and 1999. The expression of MT1-MMP was evaluated immunohistochemically using monoclonal antibodies anti-MT1-MMP (Mouse Anti-Human MT1-MMP, MAB3319 Chemicon). The polyclonal antibodies anti-CK for developing nodal micrometastases was used as well.
Positive MT1-MMP expression in 68.2% cases was observed. Immunoexpression of MT1-MMP in advanced laryngeal carcinoma as indicator for 3-year survival was noted. In addition, levels of staining correlated with number of mitoses in tumor front and plasmocytolymphatic infiltration in its environment.
The expression of MT1-MMP in tumor front appears to play an important role in determining prognosis in patients with carcinoma of the larynx.
基质金属蛋白酶（MMPs）是蛋白水解酶，通过降解细胞外基质成分促进肿瘤扩展。MPPs在各种癌症以及头颈癌的肿瘤侵袭和转移中起关键作用。本研究的目的是调查MT1-MMP在喉鳞状细胞癌中的表达情况，将这些表达水平与肿瘤和淋巴结的临床组织学特征相关联，例如TNM、淋巴结微转移、肿瘤前沿分级以及3年和5年生存率。
该研究纳入了1998年至1999年间在罗兹医科大学耳鼻喉科接受手术治疗的22例喉癌患者。使用抗MT1-MMP单克隆抗体（小鼠抗人MT1-MMP，MAB3319 Chemicon）通过免疫组织化学评估MT1-MMP的表达。还使用了抗CK多克隆抗体来检测淋巴结微转移。
观察到68.2%的病例MT1-MMP表达呈阳性。注意到MT1-MMP在晚期喉癌中的免疫表达可作为3年生存率的指标。此外，染色水平与肿瘤前沿的有丝分裂数量及其周围的浆细胞淋巴浸润相关。
MT1-MMP在肿瘤前沿的表达似乎在决定喉癌患者的预后中起重要作用。
