Wang Guei-Jane, Tsai Tung-Hu, Lin Lie-Chwen
National Research Institute of Chinese Medicine, 155-1, Li-Nong Street Section 2, Taipei 112, Taiwan.
Phytochemistry. 2007 Oct;68(19):2455-64. doi: 10.1016/j.phytochem.2007.05.035. Epub 2007 Jul 5.
Chemical examination of the n-BuOH extract from the aerial parts of Epimedium sagittatum led to isolation of three prenylated flavonol glycosides sagittasine A-C, two acylated flavonol glycosides kaempferol-3-O-(2''-E-p-coumaroyl,4''-Z-p-coumaroyl)-alpha-l-rhamnopyranoside and kaempferol-3-O-(3''-Z-p-coumaroyl,4''-E-p-coumaroyl)-alpha-l-rhamnopyranoside, together with known flavonoids, flavonolignans, 2-phenoxychromones, a lignan, and aromatic acid derivatives. Flavonolignans were identified for the first time in this plant. The vasorelaxing properties of the n-BuOH extract of E. sagittatum and 13 isolated compounds were tested using pre-contracted rat aorta rings in an organ bath apparatus. The results indicated that the n-BuOH extract of E. sagittatum produced a partial endothelial nitric oxide-dependent vasorelaxation, with EC(50) of 0.16+/-0.03 mg/ml. However, the 13 compounds tested, generated only a mild or moderate relaxation, and did not possess significant vasorelaxing effect individually.
对箭叶淫羊藿地上部分的正丁醇提取物进行化学检查，从中分离出三种异戊烯基黄酮醇苷矢车菊素A - C、两种酰化黄酮醇苷山柰酚 - 3 - O -（2'' - E - 对香豆酰基，4'' - Z - 对香豆酰基）-α - L - 鼠李糖苷和山柰酚 - 3 - O -（3'' - Z - 对香豆酰基，4'' - E - 对香豆酰基）-α - L - 鼠李糖苷，以及已知的黄酮类化合物、黄酮木脂素、2 - 苯氧基色酮、一种木脂素和芳香酸衍生物。黄酮木脂素首次在该植物中被鉴定出来。使用器官浴装置中预先收缩的大鼠主动脉环，测试了箭叶淫羊藿正丁醇提取物和13种分离化合物的血管舒张特性。结果表明，箭叶淫羊藿正丁醇提取物产生了部分依赖内皮一氧化氮的血管舒张作用，EC(50)为0.16±0.03 mg/ml。然而，所测试的13种化合物仅产生轻微或中度的舒张作用，且单独不具有显著的血管舒张效果。
