Bogut Agnieszka, Kozioł-Montewka Maria, Baranowicz Iwona, Jóźwiak Lucyna, Ksiazek Andrzej, Al-Doori Zainab, Morrison Donald, Kaczor Danuta, Paluch-Oleś Jolanta
Department of Clinical Microbiology, Medical University, Chodźki 1 Street, 20-093 Lublin, Poland.
New Microbiol. 2007 Apr;30(2):149-54.
The aim of the study was to investigate the rate of Staphylococcus aureus nasal and skin carriage in patients undergoing haemodialysis. The cultured staphylococcal isolates were subsequently characterized by molecular methods. The study group comprised 43 haemodialysed patients from whom nasal and skin swabs from the vascular access sites were collected. The identification of staphylococcal isolates and antibiotic susceptibility testing were performed on the basis of conventional diagnostic procedures. The staphylococci were further characterized using Pulsed-Field Gel Electrophoresis (PFGE). S. aureus was cultured from 12 (27.9%) patients. Only one (8.3%) patient was colonized with the microorganism both in the anterior nares and the vascular access site representing a single strain, as evidenced by PFGE analysis. Antibiotic susceptibility testing identified one (7.6%) methicillin-resistant S. aureus (MRSA) strain. PFGE typing identified several S. aureus genotypes with the lack of one specific strain responsible for colonization. However, it should be noted that among two (A and D) PFGE patterns genetically indistinguishable and closely related isolates (two isolates for each pattern) were identified. The obtained results revealed a relatively low rate of S. aureus carriage accompanied by low methicillin resistance rate and a significant genetic diversity of cultured isolates with the lack of one predominant strain responsible for colonization.
本研究的目的是调查接受血液透析患者的金黄色葡萄球菌鼻腔和皮肤携带率。随后采用分子方法对培养的葡萄球菌分离株进行特征分析。研究组包括43例接受血液透析的患者，采集了他们血管通路部位的鼻腔和皮肤拭子。根据传统诊断程序进行葡萄球菌分离株的鉴定和抗生素敏感性测试。使用脉冲场凝胶电泳（PFGE）对葡萄球菌进行进一步特征分析。12例（27.9%）患者培养出金黄色葡萄球菌。PFGE分析表明，仅1例（8.3%）患者的前鼻孔和血管通路部位均被该微生物定植，且为单一菌株。抗生素敏感性测试鉴定出1株（7.6%）耐甲氧西林金黄色葡萄球菌（MRSA）。PFGE分型鉴定出几种金黄色葡萄球菌基因型，不存在导致定植的单一特定菌株。然而，应当指出的是，在两种（A和D）PFGE模式中，鉴定出了基因无法区分且密切相关的分离株（每种模式各2株）。所得结果显示，金黄色葡萄球菌携带率相对较低，甲氧西林耐药率也较低，培养的分离株具有显著的遗传多样性，不存在导致定植的优势菌株。
