[血液中乙醇保存稳定性的研究]

[Research of on the stability of ethanol in preservation of ethanol in blood].

作者信息

Ma Dong, Zhuo Xian-Yi, Bu Jun, Xiang Ping, Shen Bao-Hua

机构信息

Institute of Forensic Sciences, Ministry of Justice, P.R.China, Shanghai 200063, China.

出版信息

Fa Yi Xue Za Zhi. 2007 Apr;23(2):117-9.

PMID:17619458

Abstract

OBJECTIVE

To focus on the main factors that may influence the stability of ethanol in blood then try to establish the best conditions for preserving ethanol in blood.

METHODS

Four crucial parameters, including temperature (-20, 4, 20 degrees C), preservative (NaF, No preservative, Na2O2), percentage of air chamber in container (0%, 25%, 50%), and ethanol concentration in blood (0.2, 0.8, 2.0 mg/mL) were investigated. Blood samples were stored under different conditions designed by orthogonal design L, (3(4))method. Quantification of ethanol in blood was carried out by headspace gas chromatography with FID detector. The results were statistically evaluated by analysis of variance.

RESULT

Significant changes of ethanol concentrations were observed in the samples stored in 20 degrees C without any reserveative, while there were no obvious changes in the samples under other storage conditions.

CONCLUSION

The best condition for keeping ethanol in blood stable is 4 degrees C with preservative and with 50% of air chamber in container. The temperature is main effective factor in the four factors.

摘要

目的

关注可能影响血液中乙醇稳定性的主要因素，进而尝试建立血液中乙醇的最佳保存条件。

方法

研究了四个关键参数，包括温度（-20℃、4℃、20℃）、防腐剂（氟化钠、无防腐剂、过氧化钠）、容器中气室百分比（0%、25%、50%）以及血液中乙醇浓度（0.2、0.8、2.0mg/mL）。采用正交设计L₉(3⁴)法设计不同条件储存血样。采用带氢火焰离子化检测器的顶空气相色谱法对血液中的乙醇进行定量分析。结果通过方差分析进行统计学评估。