Tolga Demirtaş T, Karakeçili Ayşe Gönen, Gümüşderelioğlu Menemşe
Chemical Engineering and Bioengineering Departments, Hacettepe University, Beytepe, Ankara, Turkey.
J Mater Sci Mater Med. 2008 Feb;19(2):729-35. doi: 10.1007/s10856-007-3008-7. Epub 2007 Jul 10.
The synthesis of an acrylamide-based superporous hydrogel composite (SPHC) with hydroxyapatite (HA) was realized by solution polymerization technique. The characterization studies were performed by FTIR studies, determination of swelling kinetics, measurement of mechanical properties, SEM/EDAX studies and cytocompatibility tests. The FTIR and EDAX studies revealed the incorporation of HA in superporous hydrogel (SPH) structure. The results obtained from swelling experiments showed that, although the extent of swelling was decreased after incorporation of HA in SPH structure, the time to reach the equilibrium swelling was not affected for SPHC. This result indicated that, the presence of HA did not block the capillary channels and the interconnected pore structure was maintained which were consistent with the images obtained from SEM photographs. The results obtained from mechanical tests showed that, in the presence of HA, the compression strength of the hydrogel composite was improved significantly when compared to SPH structure. The compressive modulus for the SPHC increased to 6.59 +/- 0.35 N/mm(2) whereas it was 0.63 +/- 0.04 N/mm(2) for the SPH. The cytocompatibility test which was performed by using L929 fibroblasts showed that both the SPH and SPHC materials were cytocompatible towards fibroblasts. The synthesized superporous hydrogel composite possesses suitable properties especially for bone tissue engineering applications and shall be considered as a novel scaffold.
通过溶液聚合技术实现了基于丙烯酰胺的超多孔水凝胶复合材料（SPHC）与羟基磷灰石（HA）的合成。通过傅里叶变换红外光谱（FTIR）研究、溶胀动力学测定、力学性能测量、扫描电子显微镜/能谱分析（SEM/EDAX）研究和细胞相容性测试进行了表征研究。FTIR和EDAX研究揭示了HA在超多孔水凝胶（SPH）结构中的掺入。溶胀实验得到的结果表明，尽管在SPH结构中掺入HA后溶胀程度降低，但SPHC达到平衡溶胀的时间不受影响。该结果表明，HA的存在并未阻塞毛细管通道，并且保持了相互连接的孔结构，这与从SEM照片获得的图像一致。力学测试得到的结果表明，在有HA存在的情况下，与SPH结构相比，水凝胶复合材料的抗压强度显著提高。SPHC的压缩模量增加到6.59±0.35 N/mm²，而SPH的压缩模量为0.63±0.04 N/mm²。使用L929成纤维细胞进行的细胞相容性测试表明，SPH和SPHC材料对成纤维细胞均具有细胞相容性。合成的超多孔水凝胶复合材料具有适合骨组织工程应用的特性，应被视为一种新型支架。
