Clèries L, Fernández-Pradas J M, Morenza J L
Universitat de Barcelona, Departament de Física Aplicada i Optica, Avinguda Diagonal 647, E-08028 Barcelona, Spain.
J Biomed Mater Res. 2000 Jan;49(1):43-52. doi: 10.1002/(sici)1097-4636(200001)49:1<43::aid-jbm6>3.0.co;2-g.
Three different calcium phosphate coatings of crystalline hydroxyapatite (HA), alpha- and beta-tricalcium phosphate (alpha+beta-TCP), or amorphous calcium phosphate (ACP) obtained by pulsed laser deposition on Ti-6Al-4V were incubated in a potentially osteogenic primary cell culture (rat bone marrow) in order to evaluate the amount and mode of mineralized bone matrix formation after 2 weeks with special emphasis on the type of interfacial structure that was created. Evaluation techniques included fluorescence labeling and scanning electron microscopy. The resistance to cellular resorption by osteoclasts was also studied. Bone matrix delaminated from the ACP coatings, while it remained on the HA and the alpha+beta-TCP coatings even after fracturing. A cementlike line was seen as the immediate contiguous interface with the nondegrading dense HA surface and with the surface of the remaining porous beta-TCP coating. Highly dense and crystalline HA coatings do not dissolve but are capable of establishing a strong bond with the bone matrix grown on top. Chemical and mechanical bonding were considered in this case. Cellular resorption was practically not observed on the HA coatings, but it was observed on the alpha+beta-TCP coatings. Resorption took place as dissolution that was due to the acidic microenvironment.
通过脉冲激光沉积在Ti-6Al-4V上获得的三种不同的磷酸钙涂层，即结晶羟基磷灰石（HA）、α-和β-磷酸三钙（α+β-TCP）或无定形磷酸钙（ACP），在潜在的成骨原代细胞培养物（大鼠骨髓）中孵育，以评估2周后矿化骨基质形成的量和模式，特别强调所形成的界面结构类型。评估技术包括荧光标记和扫描电子显微镜。还研究了破骨细胞对细胞吸收的抗性。骨基质从ACP涂层上分层，而即使在断裂后，它仍留在HA和α+β-TCP涂层上。在与非降解致密HA表面和剩余多孔β-TCP涂层表面的直接相邻界面处可见一条水泥样线。高度致密和结晶的HA涂层不会溶解，但能够与生长在其上的骨基质建立牢固的结合。在这种情况下考虑了化学和机械结合。在HA涂层上几乎未观察到细胞吸收，但在α+β-TCP涂层上观察到了细胞吸收。吸收是由于酸性微环境导致的溶解。
