Pagès-Manté A, Pagès-Bosch M, Majó-Masferrer N, Gómez-Couso H, Ares-Mazás E
Laboratorios Hipra S.A., Amer, Girona, Spain.
Avian Pathol. 2007 Aug;36(4):275-8. doi: 10.1080/03079450701439389.
An outbreak of disease associated with cryptosporidia on a red-legged partridge (Alectoris rufa) game farm is described. Morbidity (diarrhoea and cough) was between 60% and 70% during the first weeks of life (4 to 25 days) and mortality was higher than 50%. The results of bacteriological and virological analyses were negative. Histological examination and antigenic diagnosis by enzyme-linked immunosorbent analysis revealed the presence of Cryptosporidium spp. in respiratory and intestinal tracts. The application of polymerase chain reaction-restriction fragment length polymorphism techniques and sequencing of a Cryptosporidium oocyst wall protein gene fragment confirmed the existence of Cryptosporidium meleagridis in faecal samples. The results obtained suggest that avian cryptosporidiosis should be included among respiratory and enteric diseases routinely tested for in farmed birds.
本文描述了红腿鹧鸪（Alectoris rufa）养殖场发生的一起与隐孢子虫相关的疾病暴发。在幼雏生命的最初几周（4至25天），发病率（腹泻和咳嗽）在60%至70%之间，死亡率高于50%。细菌学和病毒学分析结果均为阴性。组织学检查和酶联免疫吸附分析抗原诊断显示呼吸道和肠道存在隐孢子虫属。聚合酶链反应-限制性片段长度多态性技术的应用以及隐孢子虫卵囊壁蛋白基因片段的测序证实粪便样本中存在火鸡隐孢子虫。所得结果表明，禽隐孢子虫病应纳入养殖鸟类常规检测的呼吸道和肠道疾病之中。
