Cuesta Alberto, Rodríguez Alejandro, Calderón Maria Victoria, Meseguer José, Esteban Maria Angeles
Department of Cell Biology, Fish Innate Immune System Group, University of Murcia, Murcia, Spain.
J Exp Zool A Ecol Genet Physiol. 2007 Sep 1;307(9):509-15. doi: 10.1002/jez.404.
Although neuroendocrine-immune system interaction has been shown in teleost fish, no study has evaluated the role of melatonin (Mel) on fish immune response even considering that it is affected by the photoperiod. Gilthead seabream (Sparus aurata L.) and sea bass (Dicentrarchus labrax L.) head-kidney leucocytes were incubated with Mel (0-control-, 20 pM-400 microM) and leucocyte viability and main innate cellular immune parameters were evaluated. Overall, seabream and sea bass head-kidney leucocytes incubated with low (similar to physiological) doses of Mel unchanged the innate immune response, whereas very high (pharmacological) dosages did. Phagocytosis was not affected by any Mel treatment while the peroxidase activity was significantly inhibited with the highest Mel concentration. In contrast, the sea bass respiratory burst activity was increased in a dose-dependent manner with 400 nM Mel or higher. Further studies are needed to clarify whether there are interactions between the fish pineal gland, and its hormone Mel, and the fish immune system.
尽管硬骨鱼中已显示存在神经内分泌-免疫系统相互作用，但尚无研究评估褪黑素（Mel）对鱼类免疫反应的作用，即便已知其受光周期影响。将金头鲷（Sparus aurata L.）和海鲈（Dicentrarchus labrax L.）的头肾白细胞与Mel（0 - 对照 - 、20 pM - 400 microM）一起孵育，并评估白细胞活力和主要先天性细胞免疫参数。总体而言，用低（类似于生理）剂量Mel孵育的金头鲷和海鲈头肾白细胞未改变先天性免疫反应，而非常高（药理）剂量则会改变。吞噬作用不受任何Mel处理的影响，而过氧化物酶活性在最高Mel浓度时受到显著抑制。相反，400 nM Mel或更高浓度时，海鲈的呼吸爆发活性呈剂量依赖性增加。需要进一步研究以阐明鱼类松果体及其激素Mel与鱼类免疫系统之间是否存在相互作用。
