Esteban M Angeles, Rodríguez Alejandro, Cuesta Alberto, Meseguer José
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
Fish Shellfish Immunol. 2005 Feb;18(2):109-24. doi: 10.1016/j.fsi.2004.06.003.
The main innate cellular immune responses of gilthead seabream (Sparus auratus L.) leucocytes were evaluated after in vitro incubation with human lactoferrin (Lf). Isolated head-kidney leucocytes were incubated with 0 (control) to 1 mg ml(-1) Lf-supplemented culture medium for 30, 120, 240 or 360 min and assayed for viability, peroxidase content, and respiratory burst, phagocytic and cytotoxic activities. Only respiratory burst activity was found to increase when using the highest Lf concentration (1 mg ml(-1)) and long incubation times (more than 120 min). Seabream were fed Lf-supplemented diets (0, control, 50, 100 or 200 mg kg(-1) diet). After 1 or 2 weeks of administration the leucocyte peroxidase content, respiratory burst, phagocytic and cytotoxic activities as a measure of cellular immune responses, as well as serum peroxidase and complement activity as a measure of humoral immune responses were evaluated. The results showed that Lf feeding at 100 mg kg(-1) diet for 1 week enhanced the cellular innate immune responses although only the cytotoxic activity did so significantly. The humoral immune response was not influenced by Lf feeding. In conclusion, Lf seems to affect innate immune cellular activity, mainly respiratory burst and natural cytotoxic activity. The possible use of Lf as an immunostimulant for farmed gilthead seabream is discussed.
在体外与人乳铁蛋白（Lf）孵育后，评估了金头鲷（Sparus auratus L.）白细胞的主要先天性细胞免疫反应。将分离的头肾白细胞与添加0（对照）至1 mg ml(-1) Lf的培养基孵育30、120、240或360分钟，并检测其活力、过氧化物酶含量以及呼吸爆发、吞噬和细胞毒性活性。仅在使用最高Lf浓度（1 mg ml(-1)）和较长孵育时间（超过120分钟）时，发现呼吸爆发活性增加。给金头鲷投喂添加Lf的饲料（0、对照、50、100或200 mg kg(-1)饲料）。给药1或2周后，评估白细胞过氧化物酶含量、呼吸爆发、吞噬和细胞毒性活性作为细胞免疫反应的指标，以及血清过氧化物酶和补体活性作为体液免疫反应的指标。结果表明，以100 mg kg(-1)饲料投喂Lf 1周可增强细胞先天性免疫反应，尽管只有细胞毒性活性显著增强。体液免疫反应不受投喂Lf的影响。总之，Lf似乎影响先天性免疫细胞活性，主要是呼吸爆发和自然细胞毒性活性。讨论了Lf作为养殖金头鲷免疫刺激剂的可能用途。
