Platano Daniela, Mesirca Pietro, Paffi Alessandra, Pellegrino Monica, Liberti Micaela, Apollonio Francesca, Bersani Ferdinando, Aicardi Giorgio
Department of Human and General Physiology, University of Bologna, Italy.
Bioelectromagnetics. 2007 Dec;28(8):599-607. doi: 10.1002/bem.20345.
We have studied the non-thermal effects of radiofrequency (RF) electromagnetic fields (EMFs) on Ba(2+) currents (I Ba 2+) through voltage-gated calcium channels (VGCC), recorded in primary cultures of rat cortical neurons using the patch-clamp technique. To assess whether low-level acute RF field exposure could modify the amplitude and/or the voltage-dependence of I Ba 2+, Petri dishes containing cultured neurons were exposed for 1-3 periods of 90 s to 900 MHz RF-EMF continuous wave (CW) or amplitude-modulated according to global system mobile communication standard (GSM) during whole-cell recording. The specific absorption rates (SARs) were 2 W/kg for CW and 2 W/kg (time average value) for GSM-modulated signals, respectively. The results obtained indicate that single or multiple acute exposures to either CW or GSM-modulated 900 MHz RF-EMFs do not significantly alter the current amplitude or the current-voltage relationship of I Ba 2+, through VGCC.
我们使用膜片钳技术，在大鼠皮层神经元原代培养物中记录，研究了射频（RF）电磁场（EMF）对通过电压门控钙通道（VGCC）的Ba(2+)电流（I Ba 2+）的非热效应。为了评估低水平急性RF场暴露是否会改变I Ba 2+的幅度和/或电压依赖性，在全细胞记录期间，将含有培养神经元的培养皿暴露于900 MHz RF-EMF连续波（CW）或根据全球移动通信系统标准（GSM）进行幅度调制的1 - 3个90秒周期。CW的比吸收率（SARs）为2 W/kg，GSM调制信号的比吸收率为2 W/kg（时间平均值）。所得结果表明，单次或多次急性暴露于CW或GSM调制的900 MHz RF-EMF，均不会显著改变通过VGCC的I Ba 2+的电流幅度或电流-电压关系。
