嗜热栖热菌中假定蛋白PF0899在1.85埃分辨率下的结构。
Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
作者信息
Kelley Laura-Lee Clancy, Dillard Bret D, Tempel Wolfram, Chen Lirong, Shaw Neil, Lee Doowon, Newton M Gary, Sugar Frank J, Jenney Francis E, Lee Han Seung, Shah Claudia, Poole Farris L, Adams Michael W W, Richardson Jane S, Richardson David C, Liu Zhi-Jie, Wang Bi-Cheng, Rose John
机构信息
Southeast Collaboratory for Structural Genomics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):549-52. doi: 10.1107/S1744309107024049. Epub 2007 Jun 11.
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
假设蛋白PF0899是一种来自嗜热古菌激烈火球菌的95个氨基酸残基的肽，它代表一个有六个成员的基因家族。激烈火球菌开放阅读框PF0899已被克隆、表达和结晶，其结构已由东南结构基因组学协作组（http://www.secsg.org）确定。该结构使用SCA2Structure流程从多个数据集解析得出，并根据收集到的最高分辨率数据集（一种假定的金衍生物）精修至1.85 Å，晶体学R因子为21.0%，R（自由）为24.0%。精修后的结构显示出与噬菌体HK97主要衣壳蛋白结构中观察到的楔形结构域有一些结构相似性，这表明PF0899可能是一种结构蛋白。
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