Wiryaman Timothy, Toor Navtej
University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
J Struct Biol X. 2022 Jan 23;6:100062. doi: 10.1016/j.yjsbx.2022.100062. eCollection 2022.
Large capsid-like nanocompartments called encapsulins are common in bacteria and archaea and contain cargo proteins with diverse functions. Advances in cryo-electron microscopy have enabled structure determination of many encapsulins in recent years. Here we summarize findings from recent encapsulin structures that have significant implications for their biological roles. We also compare important features such as the E-loop, cargo-peptide binding site, and the fivefold axis channel in different structures. In addition, we describe the discovery of a flavin-binding pocket within the encapsulin shell that may reveal a role for this nanocompartment in iron metabolism.
被称为封装菌素的大型衣壳样纳米隔室在细菌和古细菌中很常见，并且包含具有多种功能的货物蛋白。近年来，冷冻电子显微镜技术的进步使得许多封装菌素的结构得以确定。在这里，我们总结了近期封装菌素结构的研究结果，这些结果对其生物学作用具有重要意义。我们还比较了不同结构中的重要特征，如E环、货物肽结合位点和五重轴通道。此外，我们描述了在封装菌素外壳内发现的一个黄素结合口袋，这可能揭示了这个纳米隔室在铁代谢中的作用。
