Médigue C, Rouxel T, Vigier P, Hénaut A, Danchin A
Atelier de BioInformatique, Section Physique-Chimie, Institut Curie, Paris, France.
J Mol Biol. 1991 Dec 20;222(4):851-6. doi: 10.1016/0022-2836(91)90575-q.
After extracting more than 780 identified Escherichia coli genes from available data libraries, we investigated the codon usage of the corresponding coding sequences and extended the study of gene classes, thus obtained, to the nature and intensity of short nucleotide sequence selection, related to constraints operating at the nucleotide level. Using Factorial Correspondence Analysis we found that three classes ought to be included in order to match all data now available. The first two classes, as known, encompass genes expressed either continuously at a high level, or at a low level and/or rarely; the third class consists of genes corresponding to surface elements of the cell, genes coming from mobile elements as well as genes resulting in a high fidelity of DNA replication. This suggests that bacterial strains cultivated in the laboratory have been fixed by specific use of antimutator genes that are horizontally exchanged.
从现有数据库中提取出780多个已鉴定的大肠杆菌基因后，我们研究了相应编码序列的密码子使用情况，并将由此获得的基因类别研究扩展到短核苷酸序列选择的性质和强度，这与核苷酸水平上的限制作用有关。使用因子对应分析，我们发现为了匹配现有的所有数据，应该包括三类。如所知，前两类包括高水平持续表达的基因，或低水平和/或很少表达的基因；第三类由对应于细胞表面元件的基因、来自移动元件的基因以及导致DNA复制高保真度的基因组成。这表明实验室培养的细菌菌株已通过水平交换的抗突变基因的特定使用而固定下来。
