Kodikos Labs, Institut Cochin, 24 rue du Faubourg Saint-Jacques, Paris, 75014, France.
Microb Biotechnol. 2022 Jan;15(1):42-64. doi: 10.1111/1751-7915.13937. Epub 2021 Sep 27.
Living systems are studied using three complementary approaches: living cells, cell-free systems and computer-mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico studies. This design-build-test-learn iteration loop cycle between experiments and analyses combines together physiology, genetics, biochemistry and bioinformatics in a way that keeps going forward. Because computer-aided approaches are not directly constrained by the material nature of the entities of interest, we illustrate here how this virtuous cycle allows researchers to explore chemistry which is foreign to that present in extant life, from whole chassis to novel metabolic cycles. Particular emphasis is placed on the importance of evolution.
活细胞、无细胞系统和计算机介导的建模。对生命系统的理解进展使研究人员能够创造新的底盘和工业过程，其建立在将体内、体外和计算研究相结合的循环基础上。这种在实验和分析之间进行的设计-构建-测试-学习迭代循环将生理学、遗传学、生物化学和生物信息学结合在一起，不断向前推进。由于计算机辅助方法不受感兴趣实体的物质性质的直接限制，我们在这里说明这个良性循环如何使研究人员能够探索与现存生命中存在的化学物质不同的化学物质，从整个底盘到新的代谢循环。特别强调进化的重要性。
