Hsieh Yun-Hwa P, Ofori Jack A, Rao Qinchun, Bridgeman C Roger
Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, Florida 32312, USA.
J Agric Food Chem. 2007 Aug 8;55(16):6720-5. doi: 10.1021/jf070031e. Epub 2007 Jul 10.
Bovine plasma proteins are used as high-quality protein supplements in animal feed and as binders or colorants in food for human consumption. Religious observance, as well as recent fears of epidemic bovine spongiform encephalopathy, highlights the need for methods to detect bovine blood in processed food and animal feed for regulatory purposes, as the currently available methods are neither species-specific, blood-specific, nor valid for excessively heat-processed samples. This paper reports the development of monoclonal antibodies (MAbs) raised against bovine thermostable plasma proteins that display a unique species specificity pattern for plasma proteins. Immunoblotting revealed several thermostable antigenic proteins (10, 25, 40, and 60 kDa) in bovine plasma sterilized at 121 degrees C for 15 min. These MAbs can be employed individually or combined in immunoassays for analytical purposes and investigations of the chemical and biological properties of the thermostable plasma proteins identified here.
牛血浆蛋白在动物饲料中用作优质蛋白质补充剂，在人类食用的食品中用作粘合剂或色素。宗教习俗以及近期对牛海绵状脑病流行的担忧，凸显了出于监管目的检测加工食品和动物饲料中牛血的方法的必要性，因为目前可用的方法既不是物种特异性的，也不是血液特异性的，并且对过度热处理的样品无效。本文报道了针对牛热稳定血浆蛋白产生的单克隆抗体（MAb）的开发，这些抗体对血浆蛋白显示出独特的物种特异性模式。免疫印迹显示，在121℃下灭菌15分钟的牛血浆中有几种热稳定抗原蛋白（10、25、40和60 kDa）。这些单克隆抗体可单独使用或组合用于免疫分析，以分析此处鉴定的热稳定血浆蛋白的化学和生物学特性。
