Innocenti Marzia, Michelozzi Marco, Giaccherini Catia, Ieri Francesca, Vincieri Franco Francesco, Mulinacci Nadia
Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Firenze), Italy.
J Agric Food Chem. 2007 Aug 8;55(16):6596-602. doi: 10.1021/jf070257h. Epub 2007 Jul 10.
The aim of the present work was to develop a quali-quantitative investigation, using HPLC/DAD and HPLC/ESI/MS techniques, of the phenolic composition of berries collected from wild Tuscan plants of Juniperus communis L. and grown in three different geographical zones. The applied chromatographic elution method made it possible to well separate up to 16 different compounds belonging to flavonoids, such as isoscutellarein and 8-hydroxyluteolin or hypolaetin glycosides, and six biflavonoids, among them amentoflavone, hynokiflavone, cupressoflavone, and methyl-biflavones. To the best of the authors' knowledge this is the first report on the presence of these compounds in juniper berries. The flavonoidic content in the analyzed berries ranged between 1.46 and 3.79 mg/g of fresh pulp, whereas the amount of the biflavonoids was always lower, varying between 0.14 and 1.38 mg/g of fresh weight.
本研究的目的是利用高效液相色谱/二极管阵列检测法（HPLC/DAD）和高效液相色谱/电喷雾电离质谱法（HPLC/ESI/MS），对从托斯卡纳野生杜松（Juniperus communis L.）植株采集并生长于三个不同地理区域的浆果中的酚类成分进行定性定量研究。所应用的色谱洗脱方法能够很好地分离多达16种不同的黄酮类化合物，如异野黄芩素、8-羟基木犀草素或次黄嘌呤苷，以及6种双黄酮类化合物，其中包括穗花杉双黄酮、扁柏双黄酮、扁柏黄酮和甲基双黄酮。据作者所知，这是关于杜松子中这些化合物存在情况的首次报道。分析的浆果中黄酮类化合物的含量在1.46至3.79毫克/克新鲜果肉之间，而双黄酮类化合物的含量始终较低，在0.14至1.38毫克/克鲜重之间变化。
