Heo Ho Jin, Lee Hyun Jae, Kim Young Sik, Kang Sam Sik, Son Kun Ho, Seok Jeong Ho, Seo Un Kyo, Lee Choong Jae
Department of Pharmacology, College of Medicine, Chungnam National University, Daejeon, Korea.
Phytother Res. 2007 Dec;21(12):1130-4. doi: 10.1002/ptr.2222.
Scutellaria baicalensis Georgi has been used for the treatment of diverse chronic inflammatory diseases including respiratory disease in oriental medicine and its major components - baicalin, baicalein and wogonin - were reported to have various biological effects. This study investigated whether baicalin, baicalein and wogonin affect basal and ATP-induced mucin release from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin release. The results were as follows: (1) Baicalein did not affect both basal and ATP-induced mucin release significantly. (2) Baicalin and wogonin increased basal mucin release at the highest concentrations (10(-3) m). (3) However, baicalin and wogonin significantly inhibited ATP-induced mucin release. It is concluded that baicalin and wogonin can slightly increase basal mucin release whereas they can inhibit ATP-induced mucin release, by directly acting on airway mucin-secreting cells. It is suggested that baicalin and wogonin be further investigated for the possible use as mucoregulators during the treatment of chronic airway diseases.
在东方医学中，黄芩已被用于治疗包括呼吸道疾病在内的多种慢性炎症性疾病，据报道其主要成分——黄芩苷、黄芩素和汉黄芩素——具有多种生物学效应。本研究调查了黄芩苷、黄芩素和汉黄芩素是否会影响培养的气道上皮细胞中基础状态下以及ATP诱导的黏蛋白释放。使用（3）H - 葡糖胺对汇合的原代仓鼠气管表面上皮（HTSE）细胞进行代谢性放射性标记24小时，并在存在不同浓度的每种试剂的情况下追踪30分钟，以评估对（3）H - 黏蛋白释放的影响。结果如下：（1）黄芩素对基础状态下以及ATP诱导的黏蛋白释放均无显著影响。（2）黄芩苷和汉黄芩素在最高浓度（10^(-3) m）时增加基础黏蛋白释放。（3）然而，黄芩苷和汉黄芩素显著抑制ATP诱导的黏蛋白释放。结论是，黄芩苷和汉黄芩素可略微增加基础黏蛋白释放，而它们可通过直接作用于气道黏蛋白分泌细胞来抑制ATP诱导的黏蛋白释放。建议进一步研究黄芩苷和汉黄芩素在慢性气道疾病治疗中作为黏液调节剂的潜在用途。
