Hiraoka Kenichiro, Fuchiwaki Megumi, Hiraoka Kaori, Horiuchi Toshitaka, Murakami Tomoyo, Kinutani Masayuki, Kinutani Kazuo
Kinutani Women's Clinic, 2-1-4-3F, Ohtemachi, Hiroshima 730-0051, Japan.
Reprod Biomed Online. 2007 Jul;15(1):68-75. doi: 10.1016/s1472-6483(10)60694-3.
The objective of this study was to investigate whether a change in assisted hatching technique from partial opening to total removal of the zona pellucida improved the outcome of vitrified blastocyst transfer. This was a preliminary observational study conducted from November 2003 to April 2006. Partial opening using acid Tyrode's solution was performed in 45 cycles, while total removal using a laser and mechanical pipetting was performed in 57 cycles. The clinical pregnancy, implantation, and delivery rates were higher in the total removal group than in the partial opening group (67% versus 42%, P < 0.02; 55% versus 30%, P < 0.01; 56% versus 36%, P < 0.04, respectively). These results suggest that total removal of the zona pellucida is associated with higher pregnancy, implantation and delivery rates compared with partial opening for vitrified blastocyst transfer.
本研究的目的是调查将辅助孵化技术从部分打开透明带改为完全去除透明带是否能改善玻璃化囊胚移植的结局。这是一项于2003年11月至2006年4月进行的初步观察性研究。45个周期采用酸性台氏液进行部分打开透明带操作，57个周期采用激光和机械移液管进行完全去除透明带操作。完全去除透明带组的临床妊娠率、着床率和分娩率均高于部分打开透明带组（分别为67%对42%，P<0.02；55%对30%，P<0.01；56%对36%，P<0.04）。这些结果表明，与玻璃化囊胚移植的部分打开透明带相比，完全去除透明带与更高的妊娠率、着床率和分娩率相关。
