Randow Felix, Sale Julian E
M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Subcell Biochem. 2006;40:383-6. doi: 10.1007/978-1-4020-4896-8_30.
Retroviral transduction of DT40 provides an easy way to obtain population of cells stably expressing a transgene without causing cellular stress or the levels of cell death seen in transfection protocols. By employing Moloney Murine Leukemia Virus based constructs and pseudotyping the viral particles with VSV envelope glycoprotein, it is a highly efficient procedure, routinely resulting in transgene expression in the majority of cells. It is also rapid. From production of the transgene-containing virus to expression takes only four days.
逆转录病毒转导DT40细胞提供了一种简便的方法，可获得稳定表达转基因的细胞群体，且不会像转染方案那样引起细胞应激或细胞死亡。通过使用基于莫洛尼氏鼠白血病病毒的构建体并利用水疱性口炎病毒包膜糖蛋白对病毒颗粒进行假型化，这是一个高效的过程，通常能使大多数细胞表达转基因。它也很迅速。从产生含转基因的病毒到实现表达仅需四天。
