Vejbaesya Sasijit, Mahaisavariya Punkae, Luangtrakool Panpimon, Sermduangprateep Chutima
Department of Transfusion Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Rd, Bangkok 10700, Thailand.
J Med Assoc Thai. 2007 Jun;90(6):1188-92.
To determine the association of TNF alpha and NRAMP1 polymorphisms in leprosy.
The polymorphisms of TNF alpha at -238, -308, and NRAMP1 at INT4, D543N, and3' UTR were examined in 37 patients with leprosy (24 multibacillary and 13 paucibacillary) and 140 healthy controls. PCR-SSP and PCR-SSO method were used to type TNF and NRAMP1 polymorphisms.
The genotype frequency of TNF-308 G/A was significantly increased in all leprosy patients compared to the controls (p = 0.04, OR = 2.69). When leprosy types were divided, the allele frequency of TNF-308A was significantly increased in multibacillary leprosy compared to the normal controls (p = 0.04, OR = 2.93). There was no significant difference in the distribution of the genotypes and allele frequencies of TNF -238 and NRAMP1 polymorphisms between the patients and controls.
TNF-308A was associated with susceptibility to multibacillary leprosy.
确定麻风病中肿瘤坏死因子α（TNFα）和天然抗性相关巨噬细胞蛋白1（NRAMP1）基因多态性之间的关联。
对37例麻风病患者（24例多菌型和13例少菌型）和140名健康对照者检测TNFα基因 -238、-308位点以及NRAMP1基因INT4、D543N和3'非翻译区（UTR）的多态性。采用聚合酶链反应-序列特异性引物（PCR-SSP）和聚合酶链反应-序列特异性寡核苷酸（PCR-SSO）方法对TNF和NRAMP1基因多态性进行分型。
与对照组相比，所有麻风病患者中TNF -308 G/A基因型频率显著升高（p = 0.04，比值比[OR]=2.69）。当按麻风病类型划分时，多菌型麻风病患者中TNF -308A等位基因频率与正常对照组相比显著升高（p = 0.04，OR = 2.93）。患者与对照组之间TNF -238和NRAMP1基因多态性的基因型和等位基因频率分布无显著差异。
TNF -308A与多菌型麻风病易感性相关。
Zotero 插件