Kim J Y, Park H, Kwon K H, Park J Y, Baek J Y, Lee T S, Song H R, Park Y D, Lee S H
Department of Biomedical Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, South Korea.
Biomed Microdevices. 2008 Feb;10(1):11-20. doi: 10.1007/s10544-007-9105-z.
In this paper, we present a novel microfluidic system with pulsatile cell storing, cell-delivering and cell culturing functions on a single PDMS platform. For this purpose, we have integrated two reservoirs, a pulsatile pumping system containing two soft check valves, which were fabricated by in situ photopolymerization, six switch valves, and three cell culture chambers all developed through a simple and rapid fabrication process. The sample volume delivered per stroke was 120 nl and the transported volume was linearly related to the pumping frequency. We have investigated the effect of the pulsatile pneumatic micropumping on the cells during transport. For this purpose, we pumped two types of cell suspensions, one containing human breast adenocarcinoma cells (MCF-7) and the other mesenchymal stem cells (hMSCs) derived from bone marrow. The effect of pulsatile pumping on both cell types was examined by short and long-term culture experiments. Our results showed that the characteristics of both cells were maintained; they were not damaged by the pumping system. Evaluations were carried out by morphological inspection, viability assay and immunophenotyping analysis. The delivered MCF-7 cells and hMSCs spread and proliferated onto the gelatin coated cell culture chamber. This total micro cell culture system can be applied to cell-based high throughput screening and for co-culture of different cells with different volume.
在本文中，我们展示了一种新型微流控系统，该系统在单个聚二甲基硅氧烷（PDMS）平台上具有脉动式细胞存储、细胞输送和细胞培养功能。为此，我们集成了两个储液器、一个包含两个软止回阀的脉动泵送系统（通过原位光聚合制造）、六个切换阀以及三个细胞培养室，所有这些都是通过简单快速的制造工艺开发的。每冲程输送的样品体积为120纳升，输送体积与泵送频率呈线性关系。我们研究了脉动气动微泵在运输过程中对细胞的影响。为此，我们泵送了两种细胞悬液，一种含有人类乳腺腺癌细胞（MCF - 7），另一种是源自骨髓的间充质干细胞（hMSCs）。通过短期和长期培养实验研究了脉动泵送对这两种细胞类型的影响。我们的结果表明，两种细胞的特性得以保持；它们未被泵送系统损坏。通过形态学检查、活力测定和免疫表型分析进行评估。输送的MCF - 7细胞和hMSCs在涂有明胶的细胞培养室上扩散并增殖。这种全微细胞培养系统可应用于基于细胞的高通量筛选以及不同体积的不同细胞的共培养。
