Gabilondo Ane M, Hostalot Cristina, Garibi Jesús M, Meana J Javier, Callado Luis F
Department of Pharmacology, University of the Basque Country, E-48940 Leioa, Bizkaia, and Neurosurgery Service, Cruces Hospital, Spain.
Neurochem Int. 2008 Jan;52(1-2):230-4. doi: 10.1016/j.neuint.2007.05.015. Epub 2007 Jun 8.
Glial tumours are the most common type of brain neoplasm in humans. Tumour classification and grading represent key factors for patient management. However, current grading schemes are still limited by subjective histological criteria. In this context, gliosis has been linked to increases in monoamine oxidase B (MAO-B) activity. Thus, in the present study, MAO-B activity in membranes of glial tumours (n=20), meningiomas (n=12) and non-pathological human brains (n=15) was quantified by [14C]PEA oxidation. MAO-B activity was significantly greater in glioblastoma multiformes than in postmortem control brains (p<0.01) or meningiomas (p<0.001). There were no significant differences in MAO-B activity between glioblastoma multiformes (n=11) and low-grade astrocytomas (n=3) or anaplastic astrocytomas (n=6). In conclusion, the present results demonstrate a significant and selective increase in MAO-B activity in human gliomas when compared with meningiomas or non-tumoural tissue. These results suggest that the quantification of MAO-B activity may be a useful diagnostic tool for differentiating glial tumours from other types of brain tumours or surrounding normal brain tissue.
胶质肿瘤是人类最常见的脑肿瘤类型。肿瘤分类和分级是患者管理的关键因素。然而，目前的分级方案仍受主观组织学标准的限制。在这种情况下，胶质增生与单胺氧化酶B（MAO-B）活性增加有关。因此，在本研究中，通过[14C]PEA氧化对胶质肿瘤（n = 20）、脑膜瘤（n = 12）和非病理性人脑（n = 15）膜中的MAO-B活性进行了定量。多形性胶质母细胞瘤中的MAO-B活性显著高于死后对照脑（p<0.01）或脑膜瘤（p<0.001）。多形性胶质母细胞瘤（n = 11）与低级别星形细胞瘤（n = 3）或间变性星形细胞瘤（n = 6）之间的MAO-B活性没有显著差异。总之，本研究结果表明，与脑膜瘤或非肿瘤组织相比，人类胶质瘤中的MAO-B活性显著且选择性增加。这些结果表明，MAO-B活性的定量可能是区分胶质肿瘤与其他类型脑肿瘤或周围正常脑组织的有用诊断工具。
