Bacandritsos Nicolaos, Papanastasiou Iosif, Saitanis Costas, Nanetti Antonio, Roinioti Erifylli
Institute of Veterinary Research of Athens, National Agricultural Research Foundation, Neapoleos 25, Agia Paraskevi 153 10, Greece.
Vet Parasitol. 2007 Sep 1;148(2):174-8. doi: 10.1016/j.vetpar.2007.06.001. Epub 2007 Jul 10.
Oxalic acid field trails for the control of varroosis (Varroa destructor) were carried out in an apiary located on the Mt. Imittos (Attica, Greece). The colonies received four successive applications (approximately one every 16 days) with 4.2% oxalic acid (OA) and 60% sugar solution by trickling method with two alternative types of syringes (an automatic self-filling dosing and a single-use) from the broodright to broodless period. The results indicate that the first three applications (from 6th October to 25th November-broodright period) resulted in 65.3% cumulative mite mortality, while only the last application (after the 26th November-broodless period) resulted in 77.3% mite mortality. Very low outern temperatures reduce to the minimum the bee movability, which may result into a slower development of the OA efficacy. No poor colony growth or queen loss were observed even if the bee colonies were received the four successive OA applications with the last one taken place at a very low outern temperature (6.2 degrees C). The trickling method using an automatic-filling syringe seems to be a very quick way for applying oxalic acid in large apiaries (approximately 150hives/h).
在希腊阿提卡伊米托斯山的一个养蜂场进行了用草酸防治蜂螨（狄斯瓦螨）的田间试验。蜂群从育虫期到无虫期，使用两种不同类型的注射器（一种自动自灌式定量注射器和一种一次性注射器），通过滴灌法连续四次施用4.2%的草酸（OA）和60%的糖溶液（大约每16天一次）。结果表明，前三次施用（10月6日至11月25日——育虫期）导致螨虫累积死亡率达65.3%，而只有最后一次施用（11月26日之后——无虫期）导致螨虫死亡率达77.3%。极低的外界温度会使蜜蜂活动能力降至最低，这可能导致草酸药效的发挥变慢。即使蜂群在外界温度非常低（6.2摄氏度）的情况下连续四次施用草酸，也未观察到蜂群生长不良或蜂王损失的情况。使用自动灌液注射器的滴灌法似乎是在大型养蜂场（约150个蜂箱/小时）施用草酸的一种非常快捷的方法。
