García-Vicente Eduardo José, Benito-Murcia María, Martín María, Rey-Casero Ismael, Pérez Ana, González María, Alonso Juan Manuel, Risco David
Neobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, Spain.
Department of Animal Medicine, Facultad de Veterinaria, Universidad de Extremadura, Av. de la Universidad s/n, 10001 Cáceres, Spain.
Insects. 2024 Jan 17;15(1):67. doi: 10.3390/insects15010067.
The mite infests colonies and causes significant harm. Traditional treatments have become less effective because of mite resistance development and can also generate residues inside beehives. This study aimed to gauge the efficacy of a beehive-derived postbiotic in reducing viability and to explore its synergies with organic compounds. Four lactic acid bacteria (LAB) species, , , , and , were isolated and tested in a postbiotic form (preparations of inanimate microorganisms and/or their components) via bioassays. , , and notably reduced the mite viability compared to the control, and they were further tested together as a single postbiotic product (POS). Further bioassays were performed to assess the impact of the POS and its combinations with oxalic acid and oregano essential oil. The simple products and combinations (POS/Oregano, POS/Oxalic, Oregano/Oxalic, and POS/Oregano/Oxalic) decreased the mite viability. The most effective were the oxalic acid combinations (POS/Oregano/Oxalic, Oxalic/Oregano, POS/Oxalic), showing significant improvements compared to the individual products. These findings highlight the potential of combining organic products as a vital strategy for controlling infection. This study suggests that these combinations could serve as essential tools for combating the impact of mites on bee colonies.
这种螨虫会侵扰蜂群并造成严重危害。由于螨虫产生抗药性，传统治疗方法的效果已降低，而且还会在蜂箱内产生残留物。本研究旨在评估一种源自蜂箱的后生元在降低螨虫活力方面的功效，并探索其与有机化合物的协同作用。分离出四种乳酸菌（LAB），即嗜酸乳杆菌、德氏乳杆菌保加利亚亚种、植物乳杆菌和唾液乳杆菌，并通过生物测定法以后生元形式（无生命微生物和/或其成分的制剂）进行测试。与对照组相比，嗜酸乳杆菌、德氏乳杆菌保加利亚亚种和植物乳杆菌显著降低了螨虫的活力，随后将它们作为单一后生元产品（POS）一起进行进一步测试。进行了进一步的生物测定，以评估POS及其与草酸和牛至精油组合的影响。单一产品及其组合（POS/牛至、POS/草酸、牛至/草酸和POS/牛至/草酸）均降低了螨虫的活力。最有效的是草酸组合（POS/牛至/草酸、草酸/牛至、POS/草酸），与单一产品相比有显著改善。这些发现突出了将有机产品组合作为控制感染的重要策略的潜力。本研究表明，这些组合可作为对抗螨虫对蜂群影响的重要工具。
