采用高效液相色谱-电喷雾串联质谱法（HPLC-ESI-MS/MS）和核磁共振波谱法（NMR）鉴定小白菊（Tanacetum parthenium）中的抗氧化酚类化合物。
Identification of antioxidant phenolic compounds in feverfew (Tanacetum parthenium) by HPLC-ESI-MS/MS and NMR.
作者信息
Wu Changqing, Chen Feng, Wang Xi, Wu Yonnie, Dong Meidui, He Guoqing, Galyean Ronald D, He Lilin, Huang Guohui
机构信息
Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA.
出版信息
Phytochem Anal. 2007 Sep-Oct;18(5):401-10. doi: 10.1002/pca.995.
Antioxidant polyphenolic acids in the medicinal herb feverfew (Tanacetum parthenium) were isolated through in vitro bioassay-orientated antioxidant tests in response to 1,1-diphenyl-2-picrylhydrazyl (DPPH*) free radical scavenging and Fe(2+)-chelating activities. Purification of the active compounds and their structural elucidation involved a variety of techniques including open-column chromatography, HPLC, GC-MS, LC-MS and NMR. Major compounds with potent DPPH* scavenging activities were characterised as 3,5-, 4,5- and 3,4-di-O-caffeoylquinic acids (DCQAs). This is the first report of DCQAs found in feverfew.
通过针对1,1-二苯基-2-苦基肼（DPPH*）自由基清除和Fe(2+)螯合活性的体外生物测定导向抗氧化试验，从药用植物小白菊（Tanacetum parthenium）中分离出抗氧化多酚酸。活性化合物的纯化及其结构解析涉及多种技术，包括开放柱色谱法、高效液相色谱法、气相色谱-质谱联用、液相色谱-质谱联用和核磁共振。具有强大DPPH*清除活性的主要化合物被鉴定为3,5-、4,5-和3,4-二-O-咖啡酰奎宁酸（DCQAs）。这是首次在小白菊中发现DCQAs的报道。
Zotero 插件