Conde de Felipe M M, Molina J M, Rodríguez-Ponce E, Ruiz A, González J F
Parasitic Diseases Unit, Faculty of Veterinary Medicine, University of Las Palmas de G. C., Trasmontaria s/n, 35416-Arucas, Las Palmas, Spain.
J Parasitol. 2007 Jun;93(3):701-3. doi: 10.1645/GE-993R.1.
The evolution of the humoral responses of IgG and IgM against 29-35-kDa Toxoplasma gondii fractions from experimentally infected goats were studied and compared by ELISA with the use of whole T. gondii soluble extracts and 29-35-kDa electroeluted proteins as antigens. The IgM response to electroeluted proteins was detected from wk 1 to wk 3 postinfection, showing a similar evolution to that observed when T. gondii crude extracts were used as antigens. These results suggest that this group of proteins could be used for a more specific detection of anti-T. gondii IgM. In the same way, the IgG response was equivalent in both cases, although when 29-35-kDa T. gondii fractions were used as antigens, the level of specific IgGs reached a peak 2 wk before than when T. gondii crude extract was used. The detection by ELISA of anti-T. gondii IgM in goats does not seem to be affected by the presence of specific IgG in serum samples when 29-35-kDa protein fractions were used as antigens.
通过酶联免疫吸附测定法（ELISA），以完整的刚地弓形虫可溶性提取物和29 - 35 kDa电洗脱蛋白作为抗原，研究并比较了实验感染山羊针对29 - 35 kDa刚地弓形虫组分的IgG和IgM体液免疫反应的演变。在感染后第1周和第3周检测到针对电洗脱蛋白的IgM反应，其演变与以刚地弓形虫粗提物作为抗原时观察到的情况相似。这些结果表明，这组蛋白可用于更特异性地检测抗刚地弓形虫IgM。同样，两种情况下的IgG反应相当，不过当以29 - 35 kDa刚地弓形虫组分作为抗原时，特异性IgG水平比以刚地弓形虫粗提物作为抗原时提前2周达到峰值。当以29 - 35 kDa蛋白组分作为抗原时，用ELISA检测山羊血清中的抗刚地弓形虫IgM似乎不受血清样本中特异性IgG存在的影响。
