Burney D P, Lappin M R, Cooper C, Spilker M M
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Am J Vet Res. 1995 Jun;56(6):769-73.
An ELISA for detection of Toxoplasma gondii-specific IgA in feline serum was developed. A group of cats (n = 7) was inoculated orally with T gondii bradyzoites. Toxoplasma gondii-specific serum IgM, IgG, and IgA responses were followed sequentially by use of the ELISA for 34 weeks. Serum IgA was detected later than IgM or IgG, and was detected in most cats on week 34 after inoculation. None of the cats was seropositive for IgA during the oocyst-shedding period. A group of client-owned cats with suspected clinical toxoplasmosis and a group of healthy cats were tested for T gondii-specific IgA in serum. A trend toward association of T gondii-specific IgA in serum of cats with ocular disease was observed.
开发了一种用于检测猫血清中弓形虫特异性IgA的酶联免疫吸附测定法（ELISA）。一组猫（n = 7）经口接种弓形虫缓殖子。使用ELISA连续34周跟踪弓形虫特异性血清IgM、IgG和IgA反应。血清IgA的检测晚于IgM或IgG，在接种后第34周在大多数猫中检测到。在卵囊排出期，没有一只猫的IgA呈血清阳性。对一组疑似临床弓形虫病的客户拥有的猫和一组健康猫进行了血清中弓形虫特异性IgA检测。观察到患有眼部疾病的猫血清中弓形虫特异性IgA存在关联趋势。
