Wishnow R M, Lifrak E, Chen C C
J Infect Dis. 1976 Mar;133 Suppl:108-14.
The effects of Vibrio cholerae enterotoxin on steroidogenesis and on formation of adenosine 3':5'-cyclic phosphate (cyclic AMP) in two adrenal tumor cell lines were compared. Steroidogenesis was half-maximal at concentrations of 1 ng of cholera toxin/ml in the mutant OS-3 cells and 3 ng of cholera toxin/ml in the parent Y-1 cells. At the end of an 8-hr incubation, toxin-induced formation of cyclic AMP in the mutant cell line was reduced by 90%. A molar ratio of GM1 ganglioside (galactosyl-N-acetylgalactosaminyl [sialosyl] lactosyl ceramide; GGnSLC) to cholera toxin of 3:1 caused half-maximal inhibition of steroidogenesis in both cell lines. When equine antiserum to choleragenoid was added to adrenal cells 15 min after cholera toxin, there was marked inhibition of cyclic AMP formation and of steroidogenesis. Pretreatment of Y-1 cells with adrenocorticotropin rendered them unresponsive to hormonal induction of cyclic AMP formation, but these cells had an unimpaired response to cholera toxin. These studies, utilizing two adrenal cell lines, suggest important differences between the mode of action of cholera toxin and that of adrenocorticotropin in cultured adrenal tumor cells.
比较了霍乱弧菌肠毒素对两种肾上腺肿瘤细胞系中类固醇生成及3':5'-环磷酸腺苷（环磷酸腺苷）形成的影响。在突变型OS-3细胞中，霍乱毒素浓度为1 ng/ml时类固醇生成达到半数最大效应，在亲本Y-1细胞中该浓度为3 ng/ml。在8小时孵育结束时，毒素诱导突变细胞系中环磷酸腺苷的形成减少了90%。GM1神经节苷脂（半乳糖基-N-乙酰半乳糖胺基[唾液酸基]乳糖基神经酰胺；GGnSLC）与霍乱毒素的摩尔比为3:1时，对两种细胞系中的类固醇生成均产生半数最大抑制作用。霍乱毒素作用15分钟后，向肾上腺细胞中加入抗类霍乱原马抗血清，环磷酸腺苷形成及类固醇生成均受到显著抑制。用促肾上腺皮质激素预处理Y-1细胞，使其对激素诱导的环磷酸腺苷形成无反应，但这些细胞对霍乱毒素的反应未受损害。利用两种肾上腺细胞系进行的这些研究表明，在培养的肾上腺肿瘤细胞中，霍乱毒素与促肾上腺皮质激素的作用方式存在重要差异。
