Wang Zheng-Xu, Kueh Jacqueline L L, Teh Christina Hui-Leng, Rossbach Michael, Lim Linda, Li Pin, Wong Kee-Yew, Lufkin Thomas, Robson Paul, Stanton Lawrence W
Department of Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672.
Stem Cells. 2007 Sep;25(9):2173-82. doi: 10.1634/stemcells.2007-0085. Epub 2007 Jul 12.
Zfp206 (ZNF206 in human) encodes a zinc finger- and SCAN domain-containing protein that is highly expressed in pluripotent ESC. Upon differentiation of human and mouse ESC, Zfp206 expression is quickly repressed. Zfp206 was found to be expressed throughout embryogenesis but absent in adult tissues except testis. We have identified a role for Zfp206 in controlling ESC differentiation. ESC engineered to overexpress Zfp206 were found to be resistant to differentiation induced by retinoic acid. In addition, ESC with knocked-down expression of Zfp206 were more sensitive to differentiation by retinoic acid treatment. We found that Zfp206 was able to enhance expression from its own promoter and also activate transcription of the Oct4 and Nanog promoters. Our results show that Zfp206 is an embryonic transcription factor that plays a role in regulating pluripotency of embryonic stem cells.
Zfp206（人类中的ZNF206）编码一种含有锌指和SCAN结构域的蛋白质，该蛋白质在多能胚胎干细胞中高度表达。在人类和小鼠胚胎干细胞分化时，Zfp206的表达迅速受到抑制。研究发现Zfp206在整个胚胎发育过程中均有表达，但除睾丸外，在成年组织中不表达。我们已经确定了Zfp206在控制胚胎干细胞分化中的作用。经基因工程改造过表达Zfp206的胚胎干细胞被发现对维甲酸诱导的分化具有抗性。此外，Zfp206表达被敲低的胚胎干细胞对维甲酸处理诱导的分化更为敏感。我们发现Zfp206能够增强其自身启动子的表达，还能激活Oct4和Nanog启动子的转录。我们的结果表明，Zfp206是一种胚胎转录因子，在调节胚胎干细胞的多能性中发挥作用。
