锌指蛋白206（Zfp206）、八聚体结合转录因子4（Oct4）和性别决定区Y框蛋白2（Sox2）是胚胎干细胞中转录调控网络的整合组成部分。
Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells.
作者信息
Yu Hong-bing, Kunarso Galih, Hong Felicia Huimei, Stanton Lawrence W
机构信息
Department of Biological Sciences, National University of Singapore, Singapore 117542, Singapore.
出版信息
J Biol Chem. 2009 Nov 6;284(45):31327-35. doi: 10.1074/jbc.M109.016162. Epub 2009 Sep 9.
Abstract
Zfp206 (recently renamed Zscan10) encodes a zinc finger transcription factor specifically expressed in human and mouse embryonic stem cells (ESC). It has been shown that Zfp206 is required to maintain ESC in an undifferentiated, pluripotent state. Presented here are data showing that Zfp206 works together with two other transcription factors, Oct4 and Sox2, which are also essential regulators of ESC pluripotency. We show that Zfp206 binds to the Oct4 promoter and directly regulates Oct4 expression. Genome-wide mapping of Zfp206-binding sites in ESC identifies more than 3000 target genes, many of which encode transcription factors that are also targeted for regulation by Oct4 and Sox2. In addition, we show that Zfp206 physically interacts with both Oct4 and Sox2. These data demonstrate that Zfp206 is a key component of the core transcriptional regulatory network and together with Oct4 and Sox2 regulates differentiation of ESC.
摘要
Zfp206（最近更名为Zscan10）编码一种锌指转录因子，该因子在人类和小鼠胚胎干细胞（ESC）中特异性表达。研究表明，Zfp206是维持胚胎干细胞处于未分化的多能状态所必需的。本文展示的数据表明，Zfp206与另外两个转录因子Oct4和Sox2共同发挥作用，这两个转录因子也是胚胎干细胞多能性的重要调节因子。我们发现Zfp206结合到Oct4启动子上并直接调节Oct4的表达。对胚胎干细胞中Zfp206结合位点进行全基因组定位，鉴定出3000多个靶基因，其中许多基因编码的转录因子也是Oct4和Sox2的调控靶点。此外，我们还发现Zfp206与Oct4和Sox2都存在物理相互作用。这些数据表明，Zfp206是核心转录调控网络的关键组成部分，与Oct4和Sox2一起调节胚胎干细胞的分化。
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The transcription factor Zfp281 controls embryonic stem cell pluripotency by direct activation and repression of target genes.转录因子Zfp281通过直接激活和抑制靶基因来控制胚胎干细胞的多能性。
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Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells.锌指蛋白206是一种控制胚胎干细胞多能性的转录因子。
Stem Cells. 2007 Sep;25(9):2173-82. doi: 10.1634/stemcells.2007-0085. Epub 2007 Jul 12.
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J Biol Chem. 2007 Apr 27;282(17):12822-30. doi: 10.1074/jbc.M611814200. Epub 2007 Mar 7.
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Zic3 is required for maintenance of pluripotency in embryonic stem cells.Zic3是胚胎干细胞多能性维持所必需的。
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