Kurokawa Kenji, Kaito Chikara, Sekimizu Kazuhisa
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Methods Enzymol. 2007;422:233-44. doi: 10.1016/S0076-6879(06)22011-1.
Staphylococcus aureus is a pathogenic bacterium that causes abscesses, pneumonia, endocarditis, and food poisoning. S. aureus is also one of the resident flora of the endotherm and colonizes the host by skillfully evading its defense mechanism. Identification of attenuated mutants of S. aureus in an animal infection model is useful for investigating its adaptability and pathogenesis. This chapter describes a staphylococcal two-component SA0614-SA0615 system, which was identified using a silkworm larvae infection model.
金黄色葡萄球菌是一种致病细菌，可引起脓肿、肺炎、心内膜炎和食物中毒。金黄色葡萄球菌也是恒温动物的常驻菌群之一，并通过巧妙地逃避宿主的防御机制在宿主体内定殖。在动物感染模型中鉴定金黄色葡萄球菌的减毒突变体有助于研究其适应性和发病机制。本章描述了一种葡萄球菌双组分SA0614-SA0615系统，该系统是使用家蚕幼虫感染模型鉴定出来的。
