Yang Jian Ying, Wang Guo Xin, Liu Jia Li, Fan Jing Jing, Cui Sheng
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Reprod Toxicol. 2007 Nov-Dec;24(3-4):381-7. doi: 10.1016/j.reprotox.2007.05.009. Epub 2007 May 29.
The current study evaluated effects of zearalenone (ZEA) and its derivative alpha-zearalenol (alpha-ZOL) on male mouse semen quality, fertility and serum testosterone concentrations. Adult male mice were exposed to intraperitoneal (i.p.) injection of ZEA or alpha-ZOL at 0, 25, 50, and 75 mg/kg body weight (b.w.) daily for 7 days, and then mated with sexually mature untreated female mice. Semen quality, serum testosterone concentrations and fertility of treated mice were assessed. The results showed that the number of abnormal spermatozoa increased and the amount of live spermatozoa decreased significantly in males treated with ZEA at all doses. As well, a significant decrease in spermatozoa with integrated acrosome was observed in mice treated with 50 and 75 mg/kg b.w. alpha-ZOL. Significantly low pregnancy rate was observed when females were mated with ZEA or alpha-ZOL exposed males. Male mice exposed to ZEA had significant reductions in b.w. and relative epididymis weights. However, relative seminal vesicle weights were higher than those of controls. Conversely, significant increases in b.w. and relative preputial gland weight were observed in mice exposed to alpha-ZOL. Testicular and cauda epididymal sperm counts, efficiency of sperm production and serum testosterone concentrations were significantly reduced in mice treated with ZEA or alpha-ZOL at all doses in a dose-dependent manner. In conclusion, we demonstrate that ZEA or alpha-ZOL have adverse effects on reproductive system of adult male mice.
本研究评估了玉米赤霉烯酮（ZEA）及其衍生物α-玉米赤霉醇（α-ZOL）对雄性小鼠精液质量、生育能力和血清睾酮浓度的影响。成年雄性小鼠每天腹腔注射0、25、50和75mg/kg体重（b.w.）的ZEA或α-ZOL，持续7天，然后与性成熟的未处理雌性小鼠交配。评估处理后小鼠的精液质量、血清睾酮浓度和生育能力。结果显示，所有剂量ZEA处理的雄性小鼠异常精子数量增加，活精子数量显著减少。同样，50和75mg/kg b.w. α-ZOL处理的小鼠中，顶体完整的精子数量显著减少。当雌性小鼠与暴露于ZEA或α-ZOL的雄性小鼠交配时，观察到显著低的妊娠率。暴露于ZEA的雄性小鼠体重和附睾相对重量显著降低。然而，精囊相对重量高于对照组。相反，暴露于α-ZOL的小鼠体重和包皮腺相对重量显著增加。所有剂量的ZEA或α-ZOL处理的小鼠睾丸和附睾尾精子计数、精子产生效率和血清睾酮浓度均以剂量依赖性方式显著降低。总之，我们证明ZEA或α-ZOL对成年雄性小鼠的生殖系统有不良影响。
