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Mago Nashi和Tsunagi/Y14分别调控果蝇生殖系干细胞的分化和卵母细胞的特化。

Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification.

作者信息

Parma David H, Bennett Paul E, Boswell Robert E

机构信息

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.

出版信息

Dev Biol. 2007 Aug 15;308(2):507-19. doi: 10.1016/j.ydbio.2007.06.007. Epub 2007 Jun 13.

DOI:10.1016/j.ydbio.2007.06.007
PMID:17628520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3010412/
Abstract

A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprotein complex assembled on spliced mRNAs. In previous studies, reduced function alleles of mago nashi and tsunagi/Y14 were used to characterize the roles of the genes in oogenesis. Here, we investigated mago nashi and tsunagi/Y14 using null alleles and clonal analysis. Germline clones lacking mago nashi function divide but fail to differentiate. The mago nashi null germline stem cells produce clones over a period of at least 11 days, suggesting that mago nashi is not necessary for stem cell self-renewal. However, germline stem cells lacking tsunagi/Y14 function are indistinguishable from wild type. Additionally, in tsunagi/Y14 null germline cysts, centrosomes and oocyte-specific components fail to concentrate within a single cell and oocyte fate is not restricted to a single cell. Together, our results suggest not only that mago nashi is required for germline stem cell differentiation but that surprisingly mago nashi functions independently of tsunagi/Y14 in this process. On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process.

摘要

在黑腹果蝇卵子发生过程中，由Mago Nashi和Tsunagi/Y14组成的蛋白质复合物对于建立主要体轴以及原始生殖细胞决定因子的定位是必需的。Mago Nashi:Tsunagi/Y14异二聚体还作为外显子连接复合物（EJC）的核心，EJC是一种组装在剪接mRNA上的多蛋白复合物。在先前的研究中，使用mago nashi和tsunagi/Y14的功能降低等位基因来表征这些基因在卵子发生中的作用。在这里，我们使用无效等位基因和克隆分析来研究mago nashi和tsunagi/Y14。缺乏mago nashi功能的生殖系克隆能够分裂但无法分化。mago nashi无效的生殖系干细胞在至少11天的时间内产生克隆，这表明mago nashi对于干细胞自我更新不是必需的。然而，缺乏tsunagi/Y14功能的生殖系干细胞与野生型无法区分。此外，在tsunagi/Y14无效的生殖系囊肿中，中心体和卵母细胞特异性成分无法集中在单个细胞内，并且卵母细胞命运不限于单个细胞。总之，我们的结果不仅表明mago nashi是生殖系干细胞分化所必需的，而且令人惊讶的是，mago nashi在这个过程中独立于tsunagi/Y14发挥作用。另一方面，Tsunagi/Y14对于将卵母细胞命运限制在单个细胞中是必不可少的，并且在这个过程中可能与mago nashi一起发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/ea0e74c4a408/nihms-242631-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/6e6efd1fed30/nihms-242631-f0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/0f5ba0ba5768/nihms-242631-f0005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/c35d6e01ead1/nihms-242631-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/ea0e74c4a408/nihms-242631-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/6e6efd1fed30/nihms-242631-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/e715fe806778/nihms-242631-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/4a3166bc75b0/nihms-242631-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/63c29a2c858b/nihms-242631-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/0f5ba0ba5768/nihms-242631-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/8b1c49f9d6c1/nihms-242631-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/c35d6e01ead1/nihms-242631-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cfa/3010412/ea0e74c4a408/nihms-242631-f0008.jpg

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