Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
Biozentrum, Lehrstuhl für Zell-und Entwicklungsbiologie, Universität Würzburg, Am Hubland, Würzburg, Germany.
PLoS One. 2018 Mar 21;13(3):e0192633. doi: 10.1371/journal.pone.0192633. eCollection 2018.
The degree of conservation and evolution of cytoplasmic mRNA metabolism pathways across the eukaryotes remains incompletely resolved. In this study, we describe a comprehensive genome and transcriptome-wide analysis of proteins involved in mRNA maturation, translation, and mRNA decay across representative organisms from the six eukaryotic super-groups. We demonstrate that eukaryotes share common pathways for mRNA metabolism that were almost certainly present in the last eukaryotic common ancestor, and show for the first time a correlation between intron density and a selective absence of some Exon Junction Complex (EJC) components in eukaryotes. In addition, we identify pathways that have diversified in individual lineages, with a specific focus on the unique gene gains and losses in members of the Excavata and SAR groups that contribute to their unique gene expression pathways compared to other organisms.
细胞质 mRNA 代谢途径在真核生物中的保守性和进化程度仍不完全清楚。在这项研究中，我们描述了对来自六个真核超组的代表性生物的蛋白质进行了全面的基因组和转录组范围内的分析，这些蛋白质参与了 mRNA 的成熟、翻译和 mRNA 降解。我们证明了真核生物共享 mRNA 代谢的共同途径，这些途径很可能存在于最后一个真核共同祖先中，并首次显示出内含子密度与某些外显子结合复合体 (EJC) 成分在真核生物中选择性缺失之间的相关性。此外，我们还鉴定了在各个谱系中多样化的途径，特别关注挖掘生物和 SAR 生物成员中独特的基因获得和缺失，这些基因获得和缺失导致它们与其他生物相比具有独特的基因表达途径。
