Shintani Yoshinori, Ishikawa Yukio
Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
J Insect Physiol. 2007 Oct;53(10):1055-62. doi: 10.1016/j.jinsphys.2007.05.012. Epub 2007 Jun 9.
Rapid cold-hardening (RCH) and cold acclimation (ACC) were examined in eggs of the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae). When eggs incubated at 25 degrees C were transferred directly to conditions of -22 degrees C for 2h, less than 30% survived, whereas exposure to 0 degrees C for 4h prior to transfer to -22 degrees C increased survival to nearly 60%. The rapidly enhanced cold tolerance (RCH) was transient and lost rapidly after 1h at 25 degrees C. Incubation at 15.5 degrees C for 9 days (ACC) also enhanced cold tolerance. Comparison of the cold tolerance of non-treated eggs and eggs pre-treated to give RCH, ACC, or ACC+RCH allowed the relationship between the two hardening processes to be determined. At a mild subzero temperature (-10 degrees C) an RCH effect was not detected, whereas only RCH is effective at the severest subzero temperature just above the SCP (-26 degrees C). At intermediate temperatures (-16, -22 and -25 degrees C), ACC and RCH enhanced survival in combination. Therefore, the two hardening processes have different physiological bases but operate concomitantly over a wide temperature range.
在黄斑星天牛（Psacothea hilaris (Pascoe)，鞘翅目：天牛科）的卵中检测了快速冷硬化（RCH）和冷驯化（ACC）。当在25℃下孵化的卵直接转移到-22℃的条件下2小时，存活率不到30%，而在转移到-22℃之前先暴露于0℃4小时，存活率提高到近60%。快速增强的耐寒性（RCH）是短暂的，在25℃下1小时后迅速丧失。在15.5℃下孵化9天（ACC）也增强了耐寒性。比较未处理的卵与经过RCH、ACC或ACC+RCH预处理的卵的耐寒性，确定了两种硬化过程之间的关系。在温和的零下温度（-10℃）下未检测到RCH效应，而在刚好高于过冷却点（SCP）的最严酷的零下温度（-26℃）下只有RCH有效。在中间温度（-16℃、-22℃和-25℃）下，ACC和RCH联合提高了存活率。因此，这两种硬化过程具有不同的生理基础，但在很宽的温度范围内同时起作用。
