Marsh J B
J Lipid Res. 1976 Jan;17(1):85-9.
The apoproteins of serum lipoproteins and of lipoproteins present in a nonrecirculating perfusate of rat liver were compared by immunochemical, gel electrophoretic, and solubility techniques. Serum and perfusate very low density lipoprotein apoprotein composition were not different. No evidence for the presence of a lipoprotein resembling serum low density lipoprotein was obtained. However, the apoprotein composition of circulatory high density lipoprotein was quantitatively different from the secretory product in the density 1.06-1.21 range. As measured by stained sodium dodecyl sulfate gel electrophoretic patterns, the arginine-rich protein was the major secretory apoprotein while the A-I protein was the major apoprotein in circulating high density lipoprotein. A very similar pattern was seen in perfusates of orotic acid-fatty livers. It was concluded that although the liver secrets lipoproteins in the high density class, circulatory high density lipoprotein is largely a product of catabolic processes.
采用免疫化学、凝胶电泳和溶解性技术，对大鼠肝脏非再循环灌注液中的脂蛋白和血清脂蛋白的载脂蛋白进行了比较。血清和灌注液极低密度脂蛋白载脂蛋白组成并无差异。未获得存在类似血清低密度脂蛋白的脂蛋白的证据。然而，循环高密度脂蛋白的载脂蛋白组成在密度1.06 - 1.21范围内与分泌产物在数量上有所不同。通过染色的十二烷基硫酸钠凝胶电泳图谱测量，富含精氨酸的蛋白质是主要的分泌载脂蛋白，而A-I蛋白质是循环高密度脂蛋白中的主要载脂蛋白。在乳清酸脂肪肝的灌注液中也观察到了非常相似的模式。得出的结论是，尽管肝脏分泌高密度类脂蛋白，但循环高密度脂蛋白在很大程度上是分解代谢过程的产物。
