牙龈卟啉单胞菌fimA基因型的流行情况与牙周健康状况

[Prevalence of fimA genotypes of Porphyromonas gingivalis and periodontal health status].

作者信息

Zhao Lei, Wu Ya-Fei, Yang He, Meng Shu, Ou-Yang Yu-Ling

机构信息

Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2007 Jun;25(3):237-41.

PMID:17629195

Abstract

OBJECTIVE

To detect the distribution of fimA genotype of P. gingivalis in periodontally healthy adults and chronic periodontitis patients, and to investigate the relationship between the prevalence of fimA genotype of P. gingivalis and periodontal health status.

METHODS

Subgingival plaque samples were collected from 136 periodontally healthy adults and 115 chronic periodontitis patients. The occurrence of P. gingivalis was determined by P. gingivalis 16S rRNA PCR. Distribution of fimA genotype was assessed in P. gingivalis positive samples by PCR using primers pairs homologous to the different fimA genes.

RESULTS

P. gingivalis was detected in 22.1% of the healthy subjects and 81.7% of chronic periodontitis patients. A single fimA genotype was detected in most subgingival plaque samples. In P. gingivalis-positive healthy adults, the most prevalent fimA genotype of P. gingivalis was type I fimA. In contrast, a majority of chronic periodontitis patients carried type II fimA, followed by IV fimA and I b fimA. The univariate analysis illustrated that chronic periodontitis was associated with occurrences of type I fimA (OR = 0.97), I b (OR =13.26), II (OR = 36.62), III (OR = 4.57), IV (OR = 22.86), and V (OR = 1.19).

CONCLUSION

II fimA genotype of P. gingivalis followed by IV and I b were an important virulence factor that may account for the pathogenesis of chronic periodontitis, suggesting an increased pathogenic potential of these types.

摘要

目的

检测牙龈卟啉单胞菌fimA基因型在牙周健康成年人和慢性牙周炎患者中的分布情况，并探讨牙龈卟啉单胞菌fimA基因型的流行情况与牙周健康状况之间的关系。

方法

从136名牙周健康成年人和115名慢性牙周炎患者中采集龈下菌斑样本。通过牙龈卟啉单胞菌16S rRNA PCR检测牙龈卟啉单胞菌的存在情况。使用与不同fimA基因同源的引物对，通过PCR评估牙龈卟啉单胞菌阳性样本中fimA基因型的分布。

结果

在22.1%的健康受试者和81.7%的慢性牙周炎患者中检测到牙龈卟啉单胞菌。在大多数龈下菌斑样本中检测到单一的fimA基因型。在牙龈卟啉单胞菌阳性的健康成年人中，牙龈卟啉单胞菌最常见的fimA基因型是I型fimA。相比之下，大多数慢性牙周炎患者携带II型fimA，其次是IV型fimA和I b型fimA。单因素分析表明，慢性牙周炎与I型fimA（OR = 0.97）、I b型（OR = 13.26）、II型（OR = 36.62）、III型（OR = 4.57）、IV型（OR = 22.86）和V型（OR = 1.19）的出现有关。