Zhao L, Wu Y-F, Meng S, Yang H, OuYang Y-L, Zhou X-D
Key Laboratory of Oral Biomedical Engineering, Ministry of Education, Chengdu, Sichuan, China.
J Periodontal Res. 2007 Dec;42(6):511-7. doi: 10.1111/j.1600-0765.2007.00975.x.
Porphyromonas gingivalis fimbriae play a key role in colonization of the oral cavity. The fimA gene, which encodes fimbrillin (FimA), can be classified into six types (I-V and Ib) according to nucleotide sequence. In the present study, we investigated the relationship between the prevalence of P. gingivalis-specific fimA genotypes and periodontal health status in Chinese adults.
One-hundred and fifteen patients with chronic periodontitis and 136 periodontally healthy adults were selected. P. gingivalis detection, determination of fimA genotypes, and the co-existence of Actinobacillus actinomycetemcomitans and Tannerella forsythia with various fimA types, were assessed by the polymerase chain reaction. Odds ratios and 95% confidence intervals were calculated for associating the fimA-specific genes with periodontitis.
P. gingivalis was detected in 22.1% of healthy subjects and in 81.7% of the patients. A single fimA genotype was detected in most samples. In healthy adults, the most prevalent fimA genotype was type I (66.7%). However, type II was detected most frequently (43.6%) in the patient group, followed by type IV (30.9%). The frequency of co-existing A. actinomycetemcomitans and T. forsythia was highest in type II fimA-positive sites. Statistical analysis revealed that periodontitis was associated with occurrences of type I (odds ratio 0.97), Ib (odds ratio 13.26), II (odds ratio 36.62), III (odds ratio 4.57), IV (odds ratio 22.86) and V (odds ratio 1.19).
P. gingivalis type II followed by type IV were considered as disease-associated strains that account for the pathogenesis of chronic periodontitis in Chinese adults.
牙龈卟啉单胞菌菌毛在口腔定植中起关键作用。编码菌毛蛋白（FimA）的fimA基因根据核苷酸序列可分为六种类型（I - V和Ib）。在本研究中，我们调查了中国成年人中牙龈卟啉单胞菌特异性fimA基因型的流行情况与牙周健康状况之间的关系。
选取115例慢性牙周炎患者和136例牙周健康的成年人。通过聚合酶链反应评估牙龈卟啉单胞菌的检测、fimA基因型的确定以及伴放线放线杆菌和福赛坦氏菌与各种fimA类型的共存情况。计算将fimA特异性基因与牙周炎相关联的比值比和95%置信区间。
在22.1%的健康受试者和81.7%的患者中检测到牙龈卟啉单胞菌。大多数样本中检测到单一的fimA基因型。在健康成年人中，最常见的fimA基因型是I型（66.7%）。然而，在患者组中II型最常被检测到（43.6%），其次是IV型（30.9%）。在II型fimA阳性位点，伴放线放线杆菌和福赛坦氏菌共存的频率最高。统计分析显示，牙周炎与I型（比值比0.97）、Ib型（比值比13.26）、II型（比值比36.62）、III型（比值比4.57）、IV型（比值比22.86）和V型（比值比1.19）的出现相关。
II型牙龈卟啉单胞菌其次是IV型被认为是与疾病相关的菌株，它们在中国成年人慢性牙周炎的发病机制中起作用。
